S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Program in Bioengineering (협동과정-바이오엔지니어링전공) Theses (Master's Degree_협동과정-바이오엔지니어링전공)
Extracellular-matrix cryogels for cartilage tissue engineering
- 공과대학 협동과정 바이오엔지니어링전공
- Issue Date
- 서울대학교 대학원
- Cryogel; Hyaluronic acid; Chondroitin sulfate; Cartilage Tissue engineering; Poly (ethylene glycol) diacrylate; Gelatin
- 학위논문 (석사)-- 서울대학교 대학원 : 협동과정 바이오엔지니어링 전공, 2016. 2. 황석연.
- In this thesis, we investigated highly porous extracellular matrix (ECM) cryogels for cartilage tissue engineering. For ECM cryogel fabrication, methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) was co-polymerized with PEG-based or gelatin-based system via free radial initiation under freezing condition. This procedure induced ice crystal formation resulting in macroporous structures within ECM-cryogels. The developed PEG-based ECM-cryogel system exhibited average macroporosity of 75% and supported the chondrocyte infiltration. Additionally, gelatin-based ECM cryogel showed average porosity of 65%. When rabbit chondrocytes were cultured on PEG-based ECM cryogels, PEG/CS cryogels stimulated aggrecan gene expression and GAG accumulation, whereas PEG/HA cryogels stimulated type II collagen gene expression and collagen accumulation. Presence of CS in gelatin-based cryogels showed strong upregulation in all types of cartilage related genes (type II collagen, type X collagen, aggrecan, HAS2, PRG4, Link protein). Gelatin/HA cryogel showed significant increase in HAS2, link protein and type II collagen. These results demonstrated that the ECM cryogels can be utilized to promote specific ECM secretion for cartilage tissue engineering