Detailed Information

Nanoelectrokinetic Selective Preconcentration of Charged Molecules and Its Dynamics : 전하를 띤 입자의 나노전기동역학적 선택적 농축 및 역학 관계

DC Field Value Language
dc.contributor.authorJihye Choi-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 전기·정보공학부, 2016. 2. 김성재.-
dc.description.abstractA multilayer micro/nanofluidic device was presented for the selective preconcentration and online collection of charged molecules with different physicochemical properties based on ion concentration polarization phenomena (ICP). With a balance of electroosmotic drag force and electrophoretic force on the molecules, a sample mixture of sulforhodamine B and Alexa Fluor 488 could be highly preconcentrated and separated simultaneously. A repeated microchamber structure was employed to capture each dye at a desirable position. For subsequent on-chip or off-chip application, pneumatic microvalves were integrated and selectively collected the target dyes with cyclic valve operations. Using the integrated system, Alexa Fluor 488 was solely collected (with a separation resolution of 1.75) out of the mixture at a 30-fold preconcentration ratio. Furthermore, investigating the mechanism of preconcentration under ICP revealed that molecular interactions affected the location of preconcentrated plugs for satisfying local electroneutrality depending on their mobility. This integrated device would be a key component for lab on a chip applications.-
dc.description.tableofcontentsChapter 1. Introduction 1
Chapter 2. Theoretical background 8
2.1 Characteristics of ICP phenomenon 8
2.2 Conventional models for ion concentration polarization 10
2.3 Simple model for preconcentration based on force balance 16
2.3.1 Preconcentration at the boundary of depletion zone 16
2.3.2 Simultaneous separation and preconcentration 19
Chapter 3. Selective preconcentration with micro pneumatic valve system 21
3.1 Experimental setup 21
3.1.1 Strategies for selective preconcentration and online collection 21
3.1.2 Preparation of PDMS microchips 26
3.1.3 Materials and reagents 29
3.1.4 System operation method 32
3.2 Results and discussions 34
3.2.1 Demonstration of selective preconcentration 34
3.2.2 Stabilization of selective preconcentration 37
3.2.3 Quantitative analysis for separation and preconcentration efficiency 37
3.2.4 Collection of preconcentrated plugs by valve system 40
3.2.5 Three kinds of analyte 46
3.2.6 Correlation between voltage configuration and separation time 52
3.2.7 Mobility-dependent preconcentration dynamics 57
Capter 4. Conclusion and Future Work 63
Bibliography 65
Abstract in Korean 70
dc.format.extent7934403 bytes-
dc.publisher서울대학교 대학원-
dc.subjectselective preconcentration-
dc.subjectpermselective membrane-
dc.subjection concentration polarization-
dc.subjectmicro pneumatic valve-
dc.titleNanoelectrokinetic Selective Preconcentration of Charged Molecules and Its Dynamics-
dc.title.alternative전하를 띤 입자의 나노전기동역학적 선택적 농축 및 역학 관계-
dc.citation.pagesiv, 70-
dc.contributor.affiliation공과대학 전기·정보공학부-
Appears in Collections:
Files in This Item:


Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.