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Synthesis and characterization of fluorescent dyes and their applications for the enhancement of growth rate of Chlorella vulgaris : 미세조류 Chlorella vulgaris 의 성장 향상을 위한 광전환 형광 염료 합성 및 특성 분석
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김재필 | - |
| dc.contributor.author | 장혜연 | - |
| dc.date.accessioned | 2017-07-14T03:13:35Z | - |
| dc.date.available | 2017-07-14T03:13:35Z | - |
| dc.date.issued | 2017-02 | - |
| dc.identifier.other | 000000140652 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/123390 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 재료공학부, 2017. 2. 김재필. | - |
| dc.description.abstract | Four fluorescent dyes were carefully designed to convert wavelengths of light specifically favourable for microalgal growth in which a portion of white light was converted from green (500 – 580nm) to orange-red (580 – 650nm). Synthesized dyes were applied to microalgae cultivation system by two methodologies: (1) light-converting layer, and (2) light-converting medium in which the dyes were dissolved in either ethanol or water to form light-converting layer between microalgae cultures and the light source. Under converted light provided by both methods, Chlorella vulgaris cultures showed maximum increase in lipid productivity by 13.00% and 17.15%, and biomass productivity by 31.65% and 37.66% compared to the control. Overall, the cultivation results confirmed beneficial effects of modified light on microalgae cultures both on their lipid and biomass productivities. Note that both growth parameters achieved higher performance in the light-converting medium thereby the effectiveness of the cultivation method was validated. | - |
| dc.description.tableofcontents | CHAPTER I: Introduction 1
CHAPTER II: Background and Literature Review 3 1. The rise of 3rd generation biomass, microalgae, as an alternative energy source 3 2. Previous work on microalgae engineering 5 3. Microalgae cultivation and the light source 6 4. Design and synthesis of light-converting dyes 8 5. Bioreactor design and cultivation methodologies 10 6. Species selection 11 CHAPTER III: Methods – Synthesis 12 1. Synthesis 12 1.1 Chemicals 12 1.2 Synthesis of 1,7-dibromo-perylene-3,4,9,10-tetracarboxydiimide 13 1.3 Synthesis of N,N-Bis(2,6-diisopropylphenyl)-1,7-dibromo-perylene-3,4,9,10-tetracarboxydiimide 14 1.4 Synthesis of N,N-Bis(2,6-diisopropylphenyl)-1,7-diphenoxy-perylene-3,4,9,10-tetracarboxydiimide (1) 15 1.5 Synthesis of N,N′-Bis(2,6-diisopropylphenyl)-1,7-di-[4-(sulfuric acid)phenoxy]perylene-3,4,9,10-tetracarboxydiimide (2) 16 1.6 Synthesis of 1,6,7,12-tetrachloroperylene-3,4,9,10-tetracarboxydiimide 18 1.7 Synthesis of N,N-Bis(2,6-diisopropylphenyl)-1,6,7,12-tetrachloroperylene-3,4,9,10-tetracarboxydiimide 19 1.8 Synthesis of N,N-Bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-perylene-3,4,9,10-tetracarboxydiimide (1) 20 1.9 Synthesis of N,N-Bis(2,6-diisopropylphenyl)-1,6,7,12-tetra-[4-(sulfuric acid)phenoxy]perylene-3,4,9,10-tetracarboxydiimide (2) 21 1.10 Characterization 22 2. Cultivation 23 2.1 Strain and seed culture 23 2.2 Optimization of photoautotrophic conditions in batch operation 24 2.3 Application of light-converting dyes in microalgae cultivation 26 2.3.1 Methodology 1: Light-converting layer 27 2.3.2 Methodology 2: Light-converting medium 28 2.4 Scheme of experiments 30 3. Growth analysis 31 3.1 Cell density and biomass productivity 31 3.2 Lipid content and lipid productivity 32 3.3 Photosynthetic rate 33 CHAPTER IV: RESULTS AND DISCUSSION 34 Part 1: Synthesis 34 Part 2: Cultivation 41 Methodology 1: Light-converting layer 41 Methodology 2: Light-converting medium 44 CHAPTER V: CONCLUSION 49 REFERENCES 50 Appendix: Matrix-Assisted Laser Desorption Ionization – Time of Flight 52 Summary 56 | - |
| dc.format | application/pdf | - |
| dc.format.extent | 1722978 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | organic dyes | - |
| dc.subject | perylene | - |
| dc.subject | light-conversion | - |
| dc.subject | wavelength | - |
| dc.subject | growth | - |
| dc.subject | microalgae | - |
| dc.subject | cultivation | - |
| dc.subject.ddc | 620 | - |
| dc.title | Synthesis and characterization of fluorescent dyes and their applications for the enhancement of growth rate of Chlorella vulgaris | - |
| dc.title.alternative | 미세조류 Chlorella vulgaris 의 성장 향상을 위한 광전환 형광 염료 합성 및 특성 분석 | - |
| dc.type | Thesis | - |
| dc.description.degree | Master | - |
| dc.citation.pages | 56 | - |
| dc.contributor.affiliation | 공과대학 재료공학부 | - |
| dc.date.awarded | 2017-02 | - |
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