S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Theses (Ph.D. / Sc.D._치의학과)
The synthetic peptide on titanium surface enhances osteogenesis
- 치의학대학원 치의학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (박사)-- 서울대학교 대학원 : 치의학과, 2017. 2. 구영.
The oligopeptide including PHSRN and RGD sequence of fibronectin (F20) promoted cellular activity like adhesion, migration, proliferation and differentiation with various cells. In this study, the biologic effect to cellular activity of F20 on titanium (Ti) surface was investigated with the synthetic oligopeptide and evaluated as a biomolecule to improve the surface characteristics of dental implant.
Materials and Methods
The change of surface characteristics after applying synthetic F20 on machined and SLA Ti surface through adsorption was investigated with confocal laser scanning microscopy (CLSM) observation. The stromal cell line ST2 was used for cell source. The quality of coating was evaluated with fluorescence microscopy and confirmed with measuring of fluorescence intensity. SEM and CLSM observation for cell adhesion, cell migration assembly kit for cell migration, Picogreen assay for cell proliferation, real time PCR and ALP activity assay for differentiation, and immunoblot and ALP staining and real time PCR for molecular mechanism was used for evaluation, respectively.
The change of roughness with the coating of F20 was not remarkable. The surface characteristic of machined and SLA Ti was different after adsorption and observed with CLSM. Cell attachment was more enhanced on machined surface with F20 than SLA. The difference was more prominent in early stage after applying F20. Migration of ST2 cell was enhanced with F20 treatment in 6, 24 h. Cell proliferation and differentiation was stimulated with F20 apply. The extracellular signal-regulated kinase (Erk) signaling pathway was activated with F20 and confirmed with inhibitor U0126 apply.
From the findings of this study, F20 can be considered to promote osteoblast activity and stimulate differentiation through Erk signaling pathway. F20 can be a choice of biomaterials to improve surface modification of dental implant.