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Bone Formation of Submicron Poly(lactide-co-glycolide)/TGF-β2 Application on Anodized Titanium Implants by Electrospray: An Animal Study : Submicron Poly(lactide-co-glycolide)/TGF-β2를 전기분사법으로 코팅한 양극산화 타이타늄 임플란트의 골형성능: 동물실험

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치의학대학원 치의과학과
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서울대학교 대학원
recombinant human transforming growth factor- β2polylactic acid-polyglycolic acid copolymerosseointegrationanodized implant
학위논문 (박사)-- 서울대학교 대학원 : 치의과학과, 2016. 8. 김성균.
Purpose: Transforming growth factor - β2 has been shown to influence the proliferation and differentiation of osteoprogenitor cells in vitro. However, due to complexity of bone formation mechanism, effects of various growth factors on ossesintegration of dental implants are not clearly understood yet. This study aimed to evaluate the bone formation effect on osseointegration of anodized titanium implants coated with poly(D,L-lactide-co-glycolide)(PLGA)/recombinant human transforming growth factor – β2 (rhTGF-β2) submicron particles by electrospray technique in rabbit tibia model.
Materials and Methods: 48 implants were used in 12 New Zealand rabbits for in vivo study, and 14 implants were used for surface analysis. Anodized titanium implants coated with PLGA/rhTGF-β2 submicron particles by electrospray technique were tested as an experimental group (n=24) compared to anodized titanium implants as a control group (n=24) in an in vivo rabbit tibia model. Implant surface examination was done by using field emission-scanning electron microscope, and the surface roughness of the implants was measured with atomic force microscopy. 30 μm thick specimens were prepared for histomorphometric analysis, which was done at 3 weeks and 6 weeks after implants being placed. Measured bone to implant contact (BIC) and bone area (BA) were statistically analyzed using Krukal-Wallis test and Mann-Whitney U-test with p-value being adjusted by Bonferonni correction.
Results: FE-SEM analysis of implant surfaces confirmed uniform coating of PLGA/rhTGF-β2 particles. There were no statistically significant differences in surface roughness between two groups (p=2.78). Histomorphometric analysis revealed that BIC% and BA% of three best consecutive threads in rhTGF-β2 coated titanium implants in 3 weeks were statistically significant compared to control groups (p=0.045 and p=048 respectively). BIC% of experimental group of three best consecutive threads in 6 weeks was also found to be higher than control groups (p=0.033) whereas BA% of experimental group of three best consecutive threads in 6 weeks did not show statistically significant results. All of groups tested in total length of implant did not show any statistically significant differences between control and experimental groups.
Conclusion: With the electrospray technique, a uniform and submicron coating of rhTGF-β2 was able to be achieved. Effective and optimal concentration of rhTGF-β2 was identified to enhance BIC and BA during early healing period with the help of the PLGA carrier in a rabbit model.
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