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In vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum-Sensing Inhibitors : 쿼럼 센싱 억제제를 이용한 Porphyromonas gingivalis 성장 억제 및 치주 질환 예방에 대한 in vivo 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 구기태 | - |
dc.contributor.author | 조영재 | - |
dc.date.accessioned | 2017-07-14T05:46:27Z | - |
dc.date.available | 2017-07-14T05:46:27Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000142376 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125145 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 치의과학과, 2017. 2. 구기태. | - |
dc.description.abstract | Background: Autoinducer (AI)-2 has an important role in biofilm formation in the oral environment. Mature biofilms formed as a result of the cell to cell communication make it difficult to overcome periodontitis with the use of antibiotics. Previous in vitro studies suggest that quorum-sensing inhibitors (QSIs) interfere with AI-2. This study compared the QSI effects resulting from an oral inoculation of Porphyromonas gingivalis in an experimental animal model.
Methods: Forty-five male mice were divided into three groups (n = 15 each): 1) infection, 2) QSI, and 3) control. Infection and QSI groups received oral inoculation of P. gingivalis, whereas treatment with QSIs (furanone compound and D-ribose) was only performed in the QSIs group. The control group was a negative control not receiving manipulation. After 42 days, mice were sacrificed, and the distance from the alveolar bone crest (ABC) to the cemento-enamel junction (CEJ) was measured by microcomputed tomography. P. gingivalis DNA was quantified in the soft and hard tissues around the molar teeth by real-time polymerase chain reaction. Results: Distance from ABC to CEJ was significantly increased in the P. gingivalis infection group compared with the control group (P = 0.02) and significantly decreased in the QSI group compared with the infection group (P = 0.02). The QSI group contained 31.64% of the bacterial DNA count of the infection group. Conclusion: Use of QSIs in the mice infection model showed a reduction of bone breakdown and a decrease in the number of bacteria in vivo, suggesting that QSIs can be a new approach to prevention and treatment of periodontitis. | - |
dc.description.tableofcontents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 4 III. RESULTS 12 IV. DISCUSSION 20 V. CONCLUSIONS 25 VI. REFERENCES 26 국문초록 34 | - |
dc.format | application/pdf | - |
dc.format.extent | 589073 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Biofilms models animal orphyromonas gingivalis Quorum sensing ribose | - |
dc.subject.ddc | 617 | - |
dc.title | In vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum-Sensing Inhibitors | - |
dc.title.alternative | 쿼럼 센싱 억제제를 이용한 Porphyromonas gingivalis 성장 억제 및 치주 질환 예방에 대한 in vivo 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Young-Jae Cho | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 36 | - |
dc.contributor.affiliation | 치의학대학원 치의과학과 | - |
dc.date.awarded | 2017-02 | - |
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