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QTL Analysis for Morphological Traits and Capsaicinoids Contents in Capsicum annuum : 고추의 형태학적 특성 및 캡사이시노이드 함량을 조절하는 양적 형질 유전자좌 분석
DC Field | Value | Language |
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dc.contributor.advisor | 강병철 | - |
dc.contributor.author | 한고은 | - |
dc.date.accessioned | 2017-07-14T06:25:39Z | - |
dc.date.available | 2017-07-14T06:25:39Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000017962 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125558 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부, 2014. 2. 강병철. | - |
dc.description.abstract | Agriculturally important traits for breeding such as growth habit, yield, and pungency level are controlled by quantitative trait loci (QTLs) in pepper (Capsicum spp.). In this study QTLs were detected which are responsible for morphological traits and capsaicinoids contents. F6-F9 recombinant inbred lines (RILs) derived from a cross between pungent Perennial (C. annuum) and non-pungent Dempsey (C. annuum) were constructed. A total 28 morphological traits and capsaicinoids contents were evaluated for parents and RILs grown in three different environments. An ultra-high density linkage map containing 3,300 single nucleotide polymorphism (SNP) markers defined from whole genome sequencing of two parents and 120 RILs was used for QTL analyses. Using evaluated data and the linkage map, QTL analyses were performed for 27 morphological traits and capsaicinoids contents. Detected QTLs were consolidated to find the major QTLs controlling traits in multiple environments. 402 QTLs were detected for plant architecture, leaf, flower, fruit, and seed related traits. Consolidated 21 QTLs were mapped in chromosome 1, 2, 3, 4, 6, 7, 9, 10, and 11 which were found commonly from two or three environments. Among them, 4 QTL regions were associated with multiple traits. Major QTLs explained 50.9 and 61.4% of phenotypic variation for leaf length and fruit weight respectively. QTL analyses for capsaicin, dihydrocapsaicin and total capsaicinoids were performed and 72 QTLs were detected. Seven consolidated QTLs were distributed to chromosomes 1, 6, 9, and 11. QTLs in chromosome 6 controlling capsaicin and total capsaicinoids and in chromosome 11 controlling dihydrocapsaicin and total capsaicinoids were located in the same region. For major QTLs and the QTLs controlling more than two traits, SNP markers were located less than 1 cM. Therefore genes located in these QTL regions could be identified by SNP markers. Furthermore, SNP markers near the QTLs can be used to predict the values of quantitative trait after validation of the markers. | - |
dc.description.tableofcontents | ABSTRACT i
CONTENTS iv LIST OF TABLES vi LIST OF FIGURES vii LIST OF ABBREVIATIONS viii INTRODUCTION 1 LITERATURE REVIEW QTL analysis of morphological traits in Capsicum 5 Capsaicinoids in pepper 7 Measurement of capsaicinoids 9 Biosynthesis of capsaicinoids 9 QTL analysis of capsaicinoids 10 MATERIALS AND METHODS Plant materials 13 Phenotype evaluation 13 Genomic DNA extraction and the Pun1 genotype analysis 15 Measurement of capsaicinoids contents by HPLC analysis 15 Genetic mapping and QTL analysis 16 RESULTS Evaluation of morphological traits 18 Construction of linkage map 23 QTL analysis for morphological traits 23 Plant architecture 25 Leaf length and width 25 Flower traits 28 Fruit traits 28 Seed traits 29 Measurement of capsaicinoids contents 29 QTL analysis for capsaicinoids contents 32 DISCUSSION 35 REFERENCES 40 ABTRACT IN KOREAN 49 APPENDIX 51 | - |
dc.format | application/pdf | - |
dc.format.extent | 2518011 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | capsaicinoids contents | - |
dc.subject | morphological traits | - |
dc.subject | recombinant inbred line (RIL) | - |
dc.subject | single nucleotide polymorphism (SNP) | - |
dc.subject | quantitative trait locus (QTL) | - |
dc.subject.ddc | 633 | - |
dc.title | QTL Analysis for Morphological Traits and Capsaicinoids Contents in Capsicum annuum | - |
dc.title.alternative | 고추의 형태학적 특성 및 캡사이시노이드 함량을 조절하는 양적 형질 유전자좌 분석 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Koeun Han | - |
dc.description.degree | Master | - |
dc.citation.pages | viii, 62 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2014-02 | - |
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