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Biocheminal characterization of rice histone methyltransferase OsiEZ1

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Authors
권예진
Advisor
서학수
Major
농업생명과학대학 식물생산과학부
Issue Date
2016-02
Publisher
서울대학교 대학원
Keywords
histone methyltransferaseEnhancer of Zeste[E(z)]OsiEZ1Long non-coding RNAE3 SUMO ligaseOsSIZ1
Description
학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부 작물생명과학 전공, 2016. 2. 서학수.
Abstract
Flowering time is an important agronomic trait for grain production in crop. In Arabidopsis, FLOWERING LOCUS C (FLC) is known as a key controller of flowering time and its expression is epigenetically regulated by chromatin modification through a histone methyltransferase Enhancer of Zeste [E(z)] which is a core component of polycomb repressive complex 2 (PRC2). Long intronic non-coding RNA known as a COLD ASSISTED INTRONIC NONCODING RNA (COLDAIR) is also required for epigenetic repression of FLC expression through its interaction with PRC2. However, the epigenetic regulation mechanism of flowering time is not characterized well in rice. The functional loss of histone methyltransferase OsiEZ1 results in late flowering under short day (SD). Therefore, we investigated the biochemical characteristics of OsiEZ1. Result showed that OsiEZ1 directly interacted a intronic LncRNA expressed in the first intron of OsMADS56, a FLC homolog gene, named OsCOLDAIR. In addition, we found direct interaction between OsiEZ1 and rice E3 SUMO ligase OsSIZ1. These data indicate that OsiEZ1-mediated methylation of OsMADS56 via OsCOLDAIR and that of other target genes can be controlled by sumoylation through E3 SUMO ligase activity of OsiEZ1.
Language
English
URI
http://hdl.handle.net/10371/125583
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Theses (Master's Degree_식물생산과학부)
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