S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Plant Science (식물생산과학부) Theses (Master's Degree_식물생산과학부)
Biocheminal characterization of rice histone methyltransferase OsiEZ1
- 농업생명과학대학 식물생산과학부
- Issue Date
- 서울대학교 대학원
- histone methyltransferase; Enhancer of Zeste[E(z)]; OsiEZ1; Long non-coding RNA; E3 SUMO ligase; OsSIZ1
- 학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부 작물생명과학 전공, 2016. 2. 서학수.
- Flowering time is an important agronomic trait for grain production in crop. In Arabidopsis, FLOWERING LOCUS C (FLC) is known as a key controller of flowering time and its expression is epigenetically regulated by chromatin modification through a histone methyltransferase Enhancer of Zeste [E(z)] which is a core component of polycomb repressive complex 2 (PRC2). Long intronic non-coding RNA known as a COLD ASSISTED INTRONIC NONCODING RNA (COLDAIR) is also required for epigenetic repression of FLC expression through its interaction with PRC2. However, the epigenetic regulation mechanism of flowering time is not characterized well in rice. The functional loss of histone methyltransferase OsiEZ1 results in late flowering under short day (SD). Therefore, we investigated the biochemical characteristics of OsiEZ1. Result showed that OsiEZ1 directly interacted a intronic LncRNA expressed in the first intron of OsMADS56, a FLC homolog gene, named OsCOLDAIR. In addition, we found direct interaction between OsiEZ1 and rice E3 SUMO ligase OsSIZ1. These data indicate that OsiEZ1-mediated methylation of OsMADS56 via OsCOLDAIR and that of other target genes can be controlled by sumoylation through E3 SUMO ligase activity of OsiEZ1.