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Intergeneric Allotetraploid xBrassicoraphanus Fruits Display a Compound Phenotype Derived from the Parents Brassica rapa and Raphanus sativus : 속간이질사배체 배무채에서 배추와 무로부터
DC Field | Value | Language |
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dc.contributor.advisor | 허진회 | - |
dc.contributor.author | 박정은 | - |
dc.date.accessioned | 2017-07-14T06:27:42Z | - |
dc.date.available | 2017-07-14T06:27:42Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000133827 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125599 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부 원예생명공학 전공, 2016. 2. 허진회. | - |
dc.description.abstract | Hybridization and polyploidization occur in many flowering plants. In particular, hybridization between different species or genera may produce novel plant species with neomorphic phenotype. Although hybrids from interspecific or intergeneric crosses are generally hard to obtain. xBrassicoraphanus, also known as Baemoochae, was successfully produced as a newly synthesized intergeneric allotetraploid between Chinese cabbage (Brassica rapa L.) and radish (Raphanus sativus L.). One of the distinct characteristics is a compound morphology of the xBrassicoraphanus pistil and silique. The pistil and silique of xBrassicoraphanus display discrete but mixed features of both Chinese cabbage and radish. In this study, we examined the reproductive development of xBrassicoraphanus focusing on the pistil and silique formation and dehiscence. xBrassicoraphanus produces the flowers whose organ structures are similar in shape to the parents, but with a silique formation intermediate between Chinese cabbage and radish silique. For example, distal segment of the xBrassicoraphanus silique resembles that of radish. These segments are indehiscent after maturation. However, the shape of proximal segment of xBrassicaraphanus fruit coincides with that of Chinese cabbages silique. Proximal segment form dehiscence zone of valves through the replum, allowing the segments to separate. Interestingly, an unusual transverse cleft appears at the junction of proximal and distal segment, forming a joint region structure. To investigate duplicate gene alteration in fruit development, we isolated homologs of major genes in the fruit development genetic pathway of Arabidopsis. We explained fruit development by comparing gene expression levels between xBrassicoraphanus and its parents. This study is an example to show how altered expression of duplicate genes after genome hybridization makes neomorphic phenotype. | - |
dc.description.tableofcontents | INTRODUCTION 1
LITERATURE REVIEWS 4 1. Fruit development 4 2. Polyploidy 8 3. Fruit morphology in Brassicaceae 10 MATERIAL AND METHODS 12 Plant material and growth conditions 12 Light microscopy 12 Scanning Electron Microscopy 13 RNA Extraction for RNA-seq 13 RESULTS 15 Heteroarthrocarpic mature fruits of xBrassicoraphanus 15 Heteroarthrocarpic structure of xBrassicoraphanus fruit is formed before fertilization 16 Epidermal cells of B. rapa, xBrassicoraphanus, and R. sativus pistils 17 Different carpel and replum structures of B. rapa, R. sativus and xBrassicoraphanus 19 Joint region of xBrassicoraphanus carpel with higher cell density 21 Alteration of gene expressions which control gynoecium and fruit development 21 DISCUSSION 32 REFERENCES 35 ABSTRACT IN KOREAN 44 | - |
dc.format | application/pdf | - |
dc.format.extent | 1312491 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Polyploid | - |
dc.subject | xBrassicoraphanus | - |
dc.subject | Fruit development | - |
dc.subject | Intergeneric hybrid | - |
dc.subject.ddc | 633 | - |
dc.title | Intergeneric Allotetraploid xBrassicoraphanus Fruits Display a Compound Phenotype Derived from the Parents Brassica rapa and Raphanus sativus | - |
dc.title.alternative | 속간이질사배체 배무채에서 배추와 무로부터 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 45 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2016-02 | - |
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