S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Plant Science (식물생산과학부) Theses (Master's Degree_식물생산과학부)
Genetic diversity analysis of Jatropha curcas collections from different island in Indonesia
- Dr. Suk-Ha Lee
- 농업생명과학대학 식물생산과학부
- Issue Date
- 서울대학교 대학원
- Jatropha curcas; accessions; Indonesia; islands; genetic diversity; genetic differentiation
- 학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부, 2016. 8. 이석하.
- Jatropha curcas L. is a potential bioenergy crop that can produce oil that can substitute diesel oil. However, the large scale production effort is limited by the absence of improved cultivars that have high yield and oil content. Therefore, understanding the genetic information of J. curcas germplasm is important for breeding strategies to create improved new plant varieties. The aim of this study was to investigate the genetic diversity and population structure of Indonesian J. curcas populations from six islands. The genetic diversity and population structure analyses were conducted on Single Nucleotide Polymorphisms (SNPs) generated from genotyping-by- sequencing (GBS). Using Jatropha integerrima as an outgroup, 13,916 SNPs were detected. Among J. curcas accessions, which include accessions from other countries (Thailand, Philippines and China), 856 SNPs were detected, but only 297 SNPs were detected among Indonesian J. curcas populations. Phylogenetic relationship and structure analysis clustered the populations to two major groups. Group one consisted of populations from Bangka and Sulawesi, even though the two islands are 1572.59 km apart from each other. Group two consisted of populations from islands in the southern part of the country: Java, Lombok – Sumbawa, Flores and Timor. Furthermore, there were little genetic differentiation between populations (FST = 0.042), and non-significant isolation by distance (Mantel test P=0.305). The results of the study can provide valuable information on the origin of J. curcas in Indonesia and help formulate breeding and conservation strategies in the country.
Keywords: Jatropha curcas, accessions, Indonesia, islands, genetic diversity, genetic differentiation.
Student number : 2014-25176