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Development of Particle Size-Controlled Horse Bone Cements for Bone Regeneration : 골 재생을 위한 입도 크기가 조절된 말뼈로 제조된 골시멘트의 개발
DC Field | Value | Language |
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dc.contributor.advisor | 정종훈 | - |
dc.contributor.author | 장경제 | - |
dc.date.accessioned | 2017-07-14T06:36:03Z | - |
dc.date.available | 2017-07-14T06:36:03Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000026183 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125753 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 바이오시스템·소재학부, 2015. 2. 정종훈. | - |
dc.description.abstract | Since their initial development in the 1980s, calcium phosphate cements (CPCs) have been one of the most used biomaterial in clinical use for bone healing. They consisted of two main component, powder, which contains calcium phosphate materials, and liquid that contains various types of polymers for setting. So far, manipulating powder particle sizes were known for enhancement of mechanical strength of CPCs. However, poor research was investigated to verify the relationship between powder particle size of CPCs and cellular function. Consequently, in this research, CPCs with various particles size distribution were fabricated and various cells were seeded on the CPCs to reveal the association with particle size of CPCs and their cellular behaviors. First, CPCs were fabricated using horse bone powder with various powder size and chitosan solution. Their surface topography were measured numerically and showed that the higher particles sizes of starting powder led to higher surface roughness whilst smaller particle sizes led to lower surface roughness. Next, cell behaviors on the CPCs were observed. In these results, high surface roughness led to better proliferation of cells, in contrast, high surface roughness led to better osteogenic differentiation. In conclusion, regulation of particle size could control surface roughness, and subsequently changed cellular behaviors, especially in osteogenic differentiation. Changing surface roughness of CPCs would be a promising method for regulating bone regeneration. | - |
dc.description.tableofcontents | Abstract
Contents List of Figures List of Tables List of Terms and Abbreviations 1. Introduction 2. Objectives 3. Literature Review 3.1. Human bone structure 3.2. Bone graft substitute 3.3. Calcium phosphate materials as bone substitutes 3.4. Hydroxyapatite for powder phase of CPC 3.5. Sintered natural bone for hydroxyapatite sources 3.6. Particle size effects on the calcium phosphate cement 3.7. The effects of surface morphology on animal cells behavior 4. Materials and Methods 4.1. Powder preparation and characterization 4.1.1 Preparation of horse bone powders 4.1.2. Horse bone powder production and classification 4.1.3. Horse bone powder characterization 4.2. Fabrication and characterization of calcium phosphate cements with horse bone powder 4.2.1. Preparation of chitosan solution 4.2.2. Fabrication of calcium phosphate cements using horse bone powder 4.2.3 Characterization calcium phosphate cements 4.2.3.1. X-ray fluorescence spectrometry (XRF) 4.2.3.2. Compressive strength 4.2.3.3. Surface Roughness Profiler 4.3. In vitro test 4.3.1. Cell proliferation test 4.3.2. Alizarin red stainning assay 4.3.3. Western blot test 4.3.4. Morphological observation 4.4. Statistical data analysis 5. Results and Discussion 5.1. Characteristics according to the particle size of horse bone powder 5.1.1 FT-IR 5.1.2. XRD 5.1.3. XRF 5.1.4. Particle size analysis 5.2. Fabrication and characteristics according to the particle size of horse bone powder 5.2.1. Observation of chemical characterization 5.2.2. Compressive strength of calcium phosphate cements with horse bone powder 5.2.3. Surface roughness of calcium phosphate cements with horse bone powder 5.3. The results of In vitro study 5.3.1. Proliferation of cells on calcium phosphate cements 5.3.2. Mineral deposition assay using alizarin red staining 5.3.3. Observation of cell morphology on calcium phosphate cements 41 5.3.4. The results of western blot 6. Conclusions 7. References Abstract (Korean) | - |
dc.format | application/pdf | - |
dc.format.extent | 3654570 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Bone substitute | - |
dc.subject | Horse bone | - |
dc.subject | Bone powder size | - |
dc.subject | Calcium phosphate cements | - |
dc.subject | Bone tissue engineering | - |
dc.subject.ddc | 660 | - |
dc.title | Development of Particle Size-Controlled Horse Bone Cements for Bone Regeneration | - |
dc.title.alternative | 골 재생을 위한 입도 크기가 조절된 말뼈로 제조된 골시멘트의 개발 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Jang Kyoung-Je | - |
dc.description.degree | Master | - |
dc.citation.pages | xi, 56 | - |
dc.contributor.affiliation | 농업생명과학대학 바이오시스템·소재학부 | - |
dc.date.awarded | 2015-02 | - |
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