S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Master's Degree_농생명공학부)
Genipin, a metabolite of geniposide from Gardenia jasminoides, inhibits adipogenesis and improves lipid metabolism in adipocytes
게니핀의 지방세포에서의 분화 억제 및 지질대사 개선 효능
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- genipin; geniposide; gardenia fruit; obesity; adipogenesis; lipid metabolism; adipocytes; PPARγ; C/EBPα; FAS; glycerol
- 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2014. 2. 이기원.
- Genipin is an aglycone metabolite of geniposide which is derived from gardenia (Gardenia jasminoides) fruit. Geniposide is deglycosylated to genipin by β-glucosidase in animal intestine and liver. Gardenia fruit is well-known for its food coloring usage and also various health benefits such as anti-inflammatory and hypolipidemic activities as well, which give promising potential to control metabolic disorders and obesity. However, the effects of gardenia fruit and its active components involved in the development obesity have been poorly investigated. Thus, the present study was carried out to identify the effects of genipin, originated from gardenia fruit, on the obesity in the aspects of adipogenesis and lipid metabolism. The data showed that genipin, but not geniposide, inhibits adipogenesis and improves lipid metabolism significantly at 100 μM in 3T3-L1 cells, associated with the down-regulation of adipogenesis-related proteins (peroxisome proliferator-activated receptor gamma
PPARγ, CCAAT/enhancer-binding protein alpha
C/EBPα, and fatty acid synthase
FAS) expression. However, genipin did not show the suppressive effect on the phosphorylation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK). Moreover, glycerol release levels which is an indication of lipid metabolism, specifically lipolysis, was also decreased. Taken together, the results elucidate that genipin inhibits adipogenesis in 3T3-L1 preadipocytes and improves lipid metabolism in 3T3-L1 adipocytes. These results suggest that genipin might play a role as a potential chemopreventive compound for the control of body weight/fat.