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Functional conservation of MoCRZ1 in Magnaporthe oryzae and its orthologs in Fusarium graminearum and Neurospora crassa
DC Field | Value | Language |
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dc.contributor.advisor | 이용환 | - |
dc.contributor.author | 이다영 | - |
dc.date.accessioned | 2017-07-14T06:41:45Z | - |
dc.date.available | 2017-07-14T06:41:45Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000018366 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125851 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2014. 2. 이용환. | - |
dc.description.abstract | Calcium signaling is one of the most common and important signal transduction cascades present in any living organism and is said to be highly conserved in throughout evolution. In Magnaporthe oryzae, a rice blast pathogen, calcium signaling pathway is required for conidiation and infection-related development, which contributes to the pathogenicity of the fungus. M. oryzae calcineurin-responsive zinc finger 1 (MoCRZ1) is involved in the calcium-dependent signaling pathway of M. oryzae, which acts as a transcription factor. Deletion mutants of MoCRZ1 (∆Mocrz1) showed drastic reduction in conidiation, hypersensitivity to calcium and cell membrane destabilizing agents as well as dramatic reduction in virulence. In this study, the orthologs of MoCRZ1 in Fusarium graminearum (FgCRZ1) and Neurospora crassa (NcCRZ1) were identified using BLASTP. Domain analyses showed that FgCRZ1 and NcCRZ1, possess one calcineurin-docking domain and two C2H2 zinc finger domains, similar to MoCRZ1, suggesting that the orthologs interact with calcineurin and that they are transcription factors involved in calcium signaling. Transformations of FgCRZ1 or NcCRZ1 into ∆Mocrz1 complemented ∆Mocrz1 defects in conidiation, appresorial turgor generation and eventually restored the virulence of nearly non-pathogenic ∆Mocrz1. Both FgCRZ1 and NcCRZ1 were able to suppress the calcium sensitivity of ∆Mocrz1, however, only ΔMocrz1::NcCRZ1 was able to resist the cell wall disturbing agent (Congo red) and only ΔMocrz1::FgCRZ1 was tolerant to cell membrane destabilizing agent (SDS), showing selective complementation of cell wall and membrane stress intolerant phenotype of ∆Mocrz1. Taken altogether, these results suggest that FgCRZ1 and NcCRZ1 are calcineurin-responsive zinc finger transcription factors in F. graminearum and N. crassa, respectively, which are highly conserved despite of the differences of in lifestyle from which they belong, and could substitute the major roles of MoCRZ1 in conidiation, calcium sensitivity and virulence. | - |
dc.description.tableofcontents | CONTENTS
page ABSTRACT------------------------------------------------------------ i CONTENTS----------------------------------------------------------------------iii LIST OF TABLES-----------------------------------------------------vi LIST OF FIGURES vii INTRODUCTION-----------------------------------------------------1 MATERIALS AND METHODS------------------------------------7 I. Fungal strains, media and growth conditions----------------------------------7 II. Nucleic acid manipulations------------------------------------------------------8 III. Identification of FgCRZ1 and NcCRZ1---------------------------------------9 IV. Generation of complementation constructs and fungal transformations--------------------------------------------------------------------------------------------10 V. Southern hybridization analysis------------------------------------------------11 VI. Conidiation and conidiophore production assays 12 VII. Sensitivity to ions, cell wall and cell membrane disturbing agents 13 VIII. Appressorium formation, cytorrhysis, host penetration and infiltration assays 13 IX. Pathogenicity assay------------------------------------------------------------ 15 RESULTS 16 I. Identification of MoCRZ1 orthologs in F. graminearum and N. crassa 16 II. FgCRZ1 and NcCRZ1 restores conidiation defect of ∆Mocrz1 19 III. FgCRZ1 and NcCRZ1 can rescue ∆Mocrz1 from hypersensitivity to calcium stress 26 IV. FgCRZ1 and NcCRZ1 selectively complements sensitivity of ∆Mocrz1 to cell wall and membrane stress 31 V. FgCRZ1 and NcCRZ1 restores virulence in ∆Mocrz1 33 DISCUSSION 42 LITERATURE CITED 45 ABSTRACT IN KOREAN 53 | - |
dc.format | application/pdf | - |
dc.format.extent | 2990996 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Functional conservation Magnaporthe oryzae CRZ1 | - |
dc.subject | Fusarium graminearum CRZ1 | - |
dc.subject | Neurospora crassa CRZ1 | - |
dc.subject | Calcium signaling | - |
dc.subject.ddc | 630 | - |
dc.title | Functional conservation of MoCRZ1 in Magnaporthe oryzae and its orthologs in Fusarium graminearum and Neurospora crassa | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Da-Young Lee | - |
dc.description.degree | Master | - |
dc.citation.pages | vii, 52 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2014-02 | - |
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