Establishment of Analytical Method for Pesticide Multiresidue in Soil and Water using HPLC-UVD/FLD

Abstract

To ensure safety of the soil and water directly related to food safety at large, 85 pesticides multiresidue analysis using HPLC-UVD/FLD for soil and water was established. Based on 73 pesticides (3 UVD-groups, 2 FLD-groups) on the list of the NAQS (National Agricultural Products Quality Management Service) for crops and vegetable, 12 pesticides were newly added considering the type of groups and retention time. UVD-group 3 and FLD-group 1, 2 sensitivity was enhanced by modifying detection wavelengths. Soil wetting (NH4Cl) and various extraction solvents (acetonitrile, dichloromethane, ethyl acetate) were attempted for the optimization of sample treatment of soil and water. Acetonitrile+saturated solution 30 mL (for soil) and acetonitrile+NaCl 20 g (for water) in general had the best recovery result. Established method was validated with linearity, selectivity, sensitivity, statistical LOD/LOQ, working range, method LOD/LOQ, trueness and precision, measurement uncertainty and ruggedness. Also system suitability test was performed by retention factor, separation factor, number of theoretical plate, resolution and symmetry.