Publications
Detailed Information
Development of functional feed additives by encapsulation of α-tocopherol in β-cyclodextrin and co-treatment of α-linolenic acid / betaine : 알파-토코페롤의 베타-사이클로덱스트린 내 캡슐화 및 알파-리놀렌산 과 베테인 동시 처리를 통한 기능성 사료첨가제의 개발
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 최윤재 | - |
dc.contributor.author | 허윤정 | - |
dc.date.accessioned | 2017-07-14T06:43:39Z | - |
dc.date.available | 2017-07-14T06:43:39Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000025367 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125889 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2015. 2. 최윤재. | - |
dc.description.abstract | Functional materials are agents that have biological function with small
amount, such as vitamins, hormones and neurotransmitters. There are various types of functional materials, and their biological functions can be classified into anti-inflammation effect, anti-oxidation effect and methyl-group donation etc. Functional materials are on the border between food and medicine because of its high efficiency of biological functions. Especially, many types of functional materials are contained in livestock products, and by strengthening the amount of those materials, functional livestock products can be produced. Functional bioactive materials have some obstacles until entering into the body and functioning. Because of low stability in high temperature or UV light, they can be degraded during storage, delivery and processing. After intake, the low pH in stomach and enzymatic reaction can degrade the functional molecules. Furthermore, many of the functional materials such as unsaturated fatty acids or lipophilic vitamins are hardly dissolved in water, resulting in low absorption rate by intestinal epithelial barrier. The low stability and low bioavailability of the material are the key obstacles of their usage. To overcome this obstacles, we tried two strategies. The first study is encapsulating α-tocopherol in β-CD with surfactant mixture system. β-CD is popular encapsulation material which can include other hydrophobic material inside of its cyclic structure, forming inclusion complex. But β-CD encapsulation still has limited solubility, and has no way of optimization for II each guest molecule. So, we used surfactant mixture system together with β- CD encapsulation to improve the solubility and optimize encapsulation efficiency and release efficiency. The second study is about determining synergistic effect of ALA and betaine. By finding out synergistic composition of ALA and betaine, we can develop feed additives composed of two synergistic materials together. In chapter 1, α-tocopherol was encapsulated in β-CD with various ratio of tween 80 and span 80. The encapsulated α-tocopherol showed better encapsulation efficiency, better release rate, better stability, and finally enhanced accumulation of α-tocopherol in yolk when supplemented to hens. In chapter 2, the synergistic effect of ALA and betaine on myoblast proliferation and differentiation was analyzed. Single treatment of ALA and betaine had a little effect on myoblast proliferation or differentiation, but co-treatment of ALA and betaine enhanced both proliferation and differentiation of myoblasts. These results indicated that α-tocopherol encapsulation can enhance better stability and bioavailability, which can be applied to developing efficient functional feed additives. Moreover, ALA and betaine were found out to be have synergistic effect, so supplement of ALA and betaine together to animals probably have better effects than single supplement of ALA or betaine. | - |
dc.description.tableofcontents | Summary I
Contents III List of Tables and Figures VI List of Abbreviations IX Introduction1 Review of Literature 3 1. Functional feed additives∙ 3 1) Purpose of supplementation of functional feed additives3 2) Obstacles of using functional materials3 3) α-Tocopherol4 4) α-Linolenic acid(ALA)5 5) Betaine6 2. Nutrient delivery systems8 1) Background of nutrient delivery system research8 2) Cyclodextrin encapsulation11 3) Surfactant mixture system and hydrophile-lipophile balance (HLB) 13 3. Muscle growth14 1) Mechanism of muscle growth14 2) Effect of PUFA and betaine on muscle growth15IV Chapter 1. Encapsulation of α-tocopherol in β-CD with surfactant mixture 17 1. Introduction 17 2. Materials and methods 19 3. Results and discussion 27 1) Particle size and morphology27 2) Encapsulation efficiency28 3) In vitro release assay29 4) Heat stability assay31 5) UV stability assay33 6) Caco-2 permeability assay34 7) In vivo assay for ALA-supplement (in vivo assay – 1) 38 8) In vivo assay for ALA and encapsulated vitamin E-supplement (in vivo assay – 2)40 4. Summary 46 Chapter 2. Determination of synergistic effect of ALA and betaine on myoblast proliferation and differentiation 47 1. Introduction47 2. Materials and methods 48 3. Results and discussion52 1) Cell counting for proliferation assay52 2) Expression level of genes related to myoblast proliferation52V 3) Expression level of genes related to myoblast differentiation 56 4. Summary 62 Conclusion and Future Prospects 63 Literature Cited 66 Summary in Korean 74 Acknowledgement77 | - |
dc.format | application/pdf | - |
dc.format.extent | 3385787 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | α-tocopherol | - |
dc.subject | β-cyclodextrin | - |
dc.subject | surfactants | - |
dc.subject | spray drying | - |
dc.subject | α- linolenic acid | - |
dc.subject | ω-6/ω-3 balance | - |
dc.subject | betaine | - |
dc.subject | muscle growth | - |
dc.subject | feed additives | - |
dc.subject.ddc | 630 | - |
dc.title | Development of functional feed additives by encapsulation of α-tocopherol in β-cyclodextrin and co-treatment of α-linolenic acid / betaine | - |
dc.title.alternative | 알파-토코페롤의 베타-사이클로덱스트린 내 캡슐화 및 알파-리놀렌산 과 베테인 동시 처리를 통한 기능성 사료첨가제의 개발 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | X, 81 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2015-02 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.