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플라보노이드계 물질 포집을 위한 지질나노입자의 개발 : Development of lipid nanoparticle (LNP) system for encapsulation of flavonoid molecules

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Authors

박소정

Advisor
최영진
Major
농업생명과학대학 농생명공학부
Issue Date
2015-02
Publisher
서울대학교 대학원
Keywords
quercetinnaringeninhesperetinlipid nanoparticle (LNP)fully hydrogenated canola oil (FHCO)squalenesoybean lecithin.
Description
학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2015. 2. 최영진.
Abstract
Quercetin, naringenin, and hesperetin as flavonoids show biological activities including anti-oxidation, anti-inflammatory, and anti-cancer effects. However, their application in functional food is greatly restricted mainly due to poor water-solubility. Lipid nanoparticle (LNP) has been proposed as a new delivery system in order to improve the bioavailability of lipophilic flavonoid molecules. Here, the stable LNP system incorporating the flavonoids was developed. In response surface methodology concerning the hydrodynamic particle size (Z-average) and the contents of stable LNPs (yield), the content of fully hydrogenated canola oil, squalene, Tween 20, and soybean lecithin for preparing optimum blank LNPs were determined as 3.5, 1.5, 0.6, and 1.1 wt %, respectively. Therefore, LNP systems encapsulating the flavonoids (0–0.5 wt% of lipid matrix) were fabricated with the determined ratios, respectively, and then their physicochemical characteristics (yield, Z-average, PDI value, and ζ-potential) were characterized (>94%, ~150 nm, 0.14–0.18, and <-40 mV). Additionally, the highest entrapment efficiencies of quercetin, naringenin and quercetin LNPs were observed at the concentration of 0.3 wt% in lipid phase (82.8, 89.0, and 90.0%, respectively), which was contributed to their solubility in the lipid phase. These optimum LNP systems loading flavonoids were digested by simulated intestinal juice within 60 min, and released the molecules from the matrices to the aqueous medium (37°C) in 50% (v/v) ethanol within 12 h. These results could be used as the basis of further study to develop beverages with flavonoids.
Language
English
URI
https://hdl.handle.net/10371/125890
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