S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Master's Degree_농생명공학부)
Gingerenone A, A Polyphenol Present in Ginger, Attenuates Monocyte-Endothelial Adhesion by Suppressing Phosphorylation of I Kappa B Kinase (IKK)
생강의 폴리페놀 성분인 진저레논 에이의 I Kappa B Kinase 인산화 조절을 통한 면역세포 부착 저해 기전 규명
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부(바이오모듈레이션), 2015. 8. 이기원.
- In early stage of atherosclerosis, monocyte binds to endothelial layer, which causes inflammation in aorta. To prevent development of atherosclerosis, it is significant to inhibit the early pathogenesis. Ginger has been reported to have potent chemotherapeutic effects in cardiovascular diseases
however, many previous studies have focused on some specific compounds and mechanisms of action responsible remain unclear. Here, I analyzed major five ginger-derived compounds, and found that gingerenone A exhibited the strongest inhibitory effects against monocyte-endothelial adhesion. Furthermore, gingerenone A significantly suppressed expression of vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemotactic protein-1 (MCP-1), key mediators in regulation of monocytic interaction to endothelial cells. Immunoblot analysis revealed that gingerenone A inhibited phosphorylation of I Kappa B Kinase (IKK) in nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling, a major pathway related to vascular inflammation and atherosclerosis as well. Taken together, the results of present study demonstrate that gingerenone A attenuates monocyte adhesion and adhesion molecules expression in endothelial cells by suppressing phosphorylation of IKK.