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Identification of Plant Compounds that Disrupt the Insect Juvenile Hormone Receptor Complex
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 제연호 | - |
dc.contributor.author | 이석희 | - |
dc.date.accessioned | 2017-07-14T06:44:55Z | - |
dc.date.available | 2017-07-14T06:44:55Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.other | 000000066680 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125914 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2015. 7. 제연호. | - |
dc.description.abstract | Insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. Insect-specific growth regulators represent attractive control agents because of their safety to both humans and the environment. We identified plant compounds that serve as juvenile hormone antagonists (PJHANs). Using the yeast two hybrid system transformed with the mosquito JH receptor as a reporter system, we demonstrate that PJHANs affect the JH receptor, methoprene-tolerant (Met), by disrupting its complex with CYCLE or FISC, formation of which is required for mediating JH activity. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants: Lindera erythrocarpa and Solidago serotina. They are effective at causing mortality of mosquito larvae at relatively low LD50 values. Topical application of two diterpenes caused a reduction in the expression of Met target genes as well as the retardation of follicle development in mosquito ovaries. Hence, the newly discovered PJHANs may lead to the development of a new class of safe and effective pesticides. | - |
dc.description.tableofcontents | ABSTRACT I
TABLE OF CONTENTS III LIST OF TABLES V LIST OF FIGURES VI INTRODUCTION 1 LITERATURE REVIEW 4 1. Juvenile hormone 4 2. Methoprene-tolerant as a JH receptor 5 3. Juvabione and Juvenile hormone mimics from plant 7 4. Insect growth regulator 7 1. Juvenile hormone agonists 8 2. Ecdysone agonists 9 3. Chitin synthase inhibitor 10 METHODS 12 1. Yeast two-hybrid binding tests using growth complementation and β-galactosidase assay. 12 2. Screening of plant extracts. 13 3. Growth inhibition and anti-yeast activity tests. 14 4. Extraction, isolation and characterization of plant diterpenes. 14 5. Larval toxicity tests and LD50 determination. 23 6. In vivo tests of PJHANs. 23 7. RNA preparation and Real-Time RT-PCR. 24 8. Maintenance of mosquitoes and vertebrate approval. 24 RESULTS 25 1. Yeast two-hybrid binding tests using growth complementation and β-galactosidase assays. 25 2. Screening of plant extracts. 27 3. Extraction, isolation and characterization of plant diterpenes 31 4. In vivo tests of PJHANs 35 5. Comparison of antagonist activities of plant diterpenes against JH III and pyriproxyfen. 39 DISCUSSION 41 LITERATURE CITED 44 ABSTRACT IN KOREAN 50 ACKNOWLEDGEMENTS 52 | - |
dc.format | application/pdf | - |
dc.format.extent | 2767266 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Juvenile hormone | - |
dc.subject | receptor | - |
dc.subject | endocrine disruptor | - |
dc.subject.ddc | 630 | - |
dc.title | Identification of Plant Compounds that Disrupt the Insect Juvenile Hormone Receptor Complex | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | VII, 53 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2015-08 | - |
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