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Development of an Improved Selective Medium for the Detection of Shigella spp.
쉬겔라 분리 검출을 위한 향상된 기능의 선택배지 개발

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dc.contributor.advisor강동현-
dc.contributor.author이지연-
dc.date.accessioned2017-07-14T06:45:17Z-
dc.date.available2017-07-14T06:45:17Z-
dc.date.issued2015-08-
dc.identifier.other000000067432-
dc.identifier.urihttps://hdl.handle.net/10371/125922-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부(식품생명공학전공), 2015. 8. 강동현.-
dc.description.abstractShigella known as a waterborne pathogen causes outbreaks of Shigellosis. The outbreaks have been traced to contaminated water and food. To detect the Shigella present in contaminated foods is very important for helping to ensure food hygiene. There are several selective media including MacConkey agar, Xylose-Lysine Deoxycholate agar, Hektoen-Enteric agar
approved by The U.S. Food and Drug Administration (FDA) and International Organization for Standardization (ISO) for the isolation of Shigella from foods. But conventional culture media for Shigella spp. are neither specific nor sensitive. Therefore, I developed an improved selective medium (HEX, HE-Xylose medium) for the detection of Shigella spp. from foods. HEX contains lactose, sucrose, D-xylose, and salicin as a differentiation marker. The concentration of bile salts No.3 was reduced to a level of 0.3% which completely inhibited tested gram positive bacteria. All Shigella spp. tested (S. flexneri, S. dysenteriae, S. boydii, and S. sonnei) produced green colonies on HEX, while Hafnia alvei, found to be false positive for Shigella on HE agar, appeared as differentiable orange colonies on HEX. HEX supported a higher recovery of heat- or acid-injured Shigella than conventional medium. A total of 300 uninoculated and inoculated food samples were used to evaluate the specificity and sensitivity of HEX. The specificity was 13.50, 38.50, and 83.50 (%) on MacConkey agar, HE agar, and HEX, respectively. The sensitivity was 76.00 and 84.00 (%) for S. flexneri and 80.00 and 92.00 (%) for S. sonnei on HE agar and HEX, respectively. HEX had superior specificity and sensitivity compared to HE agar. Therefore HEX can be an appropriate selective and differential medium for detection of Shigella spp. from foods.
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dc.description.tableofcontentsABSTRACT.................................................................................................. III
CONTENTS....................................................................................................V
LIST OF TABLES......................................................................................VIII
LIST OF FIGURE........................................................................................X

I. INTRODUCTION........................................................................................1
II. MATERIALS AND METHODS................................................................4
2.1. Stock cultures........................................................................................4
2.2. Screening of the false-positive bacteria on HE agar from food samples…..............................................................................................4
2.3. Carbohydrates fermentation test...........................................................5
2.4. Growth of test bacteria on medium containing reduced concentration of selective agent...................................................................................5
2.4.1. Growth of gram positive bacteria................................................5
2.4.2. Comparison of background mesophilic bacterial populations on
food samples................................................................................6
2.5. Formulation and preparation of new selective medium (HEX)............7
2.6. Comparison of HEX with HE agar.......................................................7
2.7. Recovery of injured Shigella on HE agar and HEX.............................8
2.7.1. Bacterial cultures and cell suspension.........................................8
2.7.2. Recovery of heat-injured Shigella spp. .......................................9
2.7.3. Recovery of acid injured Shigella spp. .......................................9
2.8. Assessment of the performance of HEX using contaminated food samples................................................................................................10
2.8.1. Evaluation of the specificity of HEX.........................................10
2.8.2. Evaluation of the sensitivity of HEX.........................................11
2.9. Statistical analysis...............................................................................12
III. RESULTS................................................................................................13
3.1. Screening of the false-positive bacteria on HE agar from food samples ...............................................................................................13
3.2. Characteristics of carbohydrate-fermenting Shigella spp...................15
3.3. Growth of gram positive bacteria and comparison of the number of background mesophilic bacteria in food samples on medium containing reduced concentrations of selective agent........................17
3.4. Comparison of HEX with HE agar.....................................................20
3.5. Recovery of heat-injured Shigella spp. ..............................................25
3.6. Recovery of acid injured Shigella spp. ..............................................27
3.7. Assessment of the performance of HEX using uninoculated and inoculated food samples......................................................................29
IV. DISCUSSIONS.......................................................................................32
V. REFERENCES….....................................................................................38
VI. 국문초록................................................................................................46
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dc.formatapplication/pdf-
dc.format.extent710175 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectShigella spp.-
dc.subjectSelective and differential media-
dc.subjectFood safety-
dc.subjectDetection of foodborne pathogens-
dc.subject.ddc630-
dc.titleDevelopment of an Improved Selective Medium for the Detection of Shigella spp.-
dc.title.alternative쉬겔라 분리 검출을 위한 향상된 기능의 선택배지 개발-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages48-
dc.contributor.affiliation농업생명과학대학 농생명공학부-
dc.date.awarded2015-08-
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Theses (Master's Degree_농생명공학부)
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