S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Master's Degree_농생명공학부)
Validation of particulate TLR agonists as vaccine adjuvants for immunization efficiency of M5BT, multi-epitope subunit vaccine
구제역 바이러스 방어를 위한 멀티-에피톱 아단위 백신 M5BT의 면역 증강제로서 미립자화 TLR 아고니스트의 효과 검증
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- Foot-and-mouth disease; Adjuvant; Multi-epitope vaccine; TLR agonist; CpG ODN; poly I:C; synergistic effect
- 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2017. 2. 최윤재.
- Multi-epitope vaccine is one of the strategies to cope with Foot-and-mouth disease which has significant global impact. However, low immunogenicity derived from its protein structure requires aids of adjuvant. CpG ODN and poly I:C are agonists recognized by certain toll-like receptor expressed on immune cells and thereby, modulate innate and adaptive immunity.
In this study, first, modified medium was suggested for vaccine production. It was confirmed E.coli cultured in modified terrific broth-2 (T2B) showed improved cell and protein yield.
Next, the synergistic adjuvant effect of CpG ODN and poly I:C was evaluated by cell viability, cytokine release and antigen-specific T and B cell immune response through particle formation between vaccine and adjuvant via ionic interaction. Consequently, the adjuvanticity of two TLR agonists had positive effects on cell proliferation, pro-and anti-inflammatory cytokine increase and elicited desirable T and B cell immunity.
Therefore, this study was suggested as advanced subunit vaccine strategy combined with efficient culture system and adjuvant partner against FMD infection.