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Beneficial Effects on Skin Hydration and Anti-wrinkle by the Water Extract of Roasted Omija (Schisandra chinensis) : 로스팅 오미자 열수 추출물의 보습 효능과 피부 주름 개선 효능
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이기원 | - |
dc.contributor.author | 이하예라 | - |
dc.date.accessioned | 2017-07-14T06:49:02Z | - |
dc.date.available | 2019-04-18 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000142005 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125999 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2017. 2. 이기원. | - |
dc.description.abstract | Omija (Schisandra chinensis) has been constantly consumed in Asian countries. Donguibogam as well as many studies support that Omija has beneficial effects on skin health. It is reported that several compounds in Omija have anti-oxidative activity and anti-wrinkle effects. Most of those beneficial compounds of Omija are organic, therefore gives low solubility in water extracting method, the most common processing method in industry. Roasting changes high molecules into the small molecules by pyrolysis, facilitating the elution of useful compounds. Additionally, roasting can change the compounds into more effective form.
In this study, I hypothesized that the water extract of roasted Omija (WRO) has more effect on skin health such as hydration and anti-wrinkle formation, compared to the water extract of Omija (WO). The WRO enhances anti-oxidant activity, increases total phenolic phytochemicals and flavonoid contents, while WO shows less effect. In human keratinocyte (HaCaT) cells, the WRO alleviated solar ultraviolet (sUV)-induced matrix metalloproteinase (MMP)-1 protein and mRNA expression, while WO shows less effect. Also, WRO suppressed the transactivation of AP-1 which crucial transcription factor of MMP-1. Additionally, WRO diminished solar ultraviolet-induced mitogen activated protein kinases (MAPKs) which regulate Activator protein-1 (AP-1) transactivation. Also, WRO increases the expression of Hyaluronan synthase 2 (HAS2), which plays a critical role in the hyaluronic acid (HA) synthesis. In summary, WRO have the benefit effects on promoting anti-photoaging against solar ultraviolet-induced skin photoaging and hydration. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS 4 Chemicals and reagents 4 Sample preparation 5 Radical scavenging activity 5 Total phenolic content 6 Total flavonoid content 7 Cell culture and treatments 7 sUV irradiation 8 Cell viability 8 Western blot 9 Gelatin zymography 10 Real-time quantitative PCR 11 Enzyme-linked immunosorbent assay (ELISA) 12 Luciferase reporter gene assay 13 Statistical analysis 14 RESULTS 16 The roasting enhances anti-oxidant activity, amount of phenolic phytochemicals and flavonoid content 16 The water extract of roasted Omija decreased sUV-induced MMP-1 protein, mRNA level and gene transactivation 23 The water extract of roasted omija suppressed the AP-1 tansactivation 25 The water extract of roasted Omija diminished sUV-induced the phosphorylation ERK pathway in HaCaT cells 28 The water extract of roasted Omija increases hyaluronic acid contents and HAS 2 expression 26 DISCUSSION 30 REFERENCES 34 국문 초록 39 | - |
dc.format | application/pdf | - |
dc.format.extent | 1198416 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | 오미자 | - |
dc.subject | 로스팅 | - |
dc.subject | 주름 | - |
dc.subject | 보습 | - |
dc.subject | HAS2 | - |
dc.subject | MMP-1 | - |
dc.subject | 인간 각질형성세포 | - |
dc.subject.ddc | 630 | - |
dc.title | Beneficial Effects on Skin Hydration and Anti-wrinkle by the Water Extract of Roasted Omija (Schisandra chinensis) | - |
dc.title.alternative | 로스팅 오미자 열수 추출물의 보습 효능과 피부 주름 개선 효능 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Hayera Lee | - |
dc.description.degree | Master | - |
dc.citation.pages | vi, 42 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2017-02 | - |
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