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Gel-Sol transition of peptides derived from α-synuclein
알파-시뉴클레인으로부터 유도한 펩타이드의 젤-졸전이현상

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Authors
박재형
Advisor
백승렬
Major
공과대학 화학생물공학부
Issue Date
2014-02
Publisher
서울대학교 대학원
Keywords
α-synucleinAmyloidTandem Repeat SequenceSelf-assemblyGel-Sol transition
Description
학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2014. 2. 백승렬.
Abstract
α-synuclein is a 140 residue protein, expressed in neurons. Through the specific self-assembly process, α-synuclein make insoluble protein aggregates such as Lewy bodies, the common characteristic of Parkinsons disease. The self-assembly of α-synuclein becomes popular because of its pathological reason and also non disease functional role. Using TANGO program to predict amyloidogenic propensity, we found a specific sequence, based on α-synuclein sequence (35-40) , EGVLYV. Although this sequence is not located in NAC region (61-95), core region of the fibrillation process of α-synuclein, when this sequence becomes tandem repeat, EGVLYV-EGVLYV, the modified peptide shows Gel-Sol transition. The self-assembly of peptide from α-synuclein is studied in DMSO. The self-assembled structure (gel state) is transformed into sol state by applying proper force. When force is removed, the Sol state structure becomes Gel state. The assembled of peptide shows honey comb structure, confirmed by scanning electron microscopy. The characterization of Gel-Sol transition is proved by Advanced Rheometric Expansion System (ARES). The structure differences between Sol state and Gel state are proved by circular dichroism, and FT-IR. Comparing with other peptide gel, the peptide structure has a unique property such as Gel-Sol transition. Mechanical force sensitive self-assembled peptide is made by using peptide derived from α-synuclein. This novel peptide will be used to make mechanical sensitive materials.
Language
English
URI
https://hdl.handle.net/10371/127100
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Theses (Master's Degree_화학생물공학부)
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