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Effects of TMR and Separate Feeding System on Ruminal Methane Production, Total Digestibility, Rumen Metabolic and Microbial Profile

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dc.contributor.advisorKim Kyoung Hoon-
dc.contributor.authorRajaramanBharanidharan-
dc.date.accessioned2017-07-17T08:54:55Z-
dc.date.available2017-07-17T08:54:55Z-
dc.date.issued2017-02-
dc.identifier.other000000141792-
dc.identifier.urihttps://hdl.handle.net/10371/127131-
dc.description학위논문 (석사)-- 서울대학교 국제농업기술대학원 : 국제농업기술학과, 2017. 2. 김경훈.-
dc.description.abstractP < 0.005) compared to those fed TMR. Also, cattle those received SF exhibited significantly lower (P < 0.05) ruminal pH and higher (P < 0.05) ammonia-N concentration and total VFA compared to those fed TMR. A significantly (P < 0.05) lower acetate: propionate ratio (2.2 vs. 2.6) in those fed SF reflected the shift in hydrogen transfer towards the formation of more propionate than those fed TMR. Significantly higher levels of isobutyrate and isovalerate (P < 0.05) were observed in those fed SF compared to those fed TMR. The total tract digestibility of CP, NDF, and OM were not affected by the feeding system. 16SrRNA analysis revealed the changes in the representation of bacteria belonging to the phyla Bacteroidetes and Firmicutes between the feeding system. TMR feeding system significantly increased (P < 0.1) the Leuconostoc and RFN20 population. SF system exhibited higher abundance of Coprococcus. Overall, these results indicate that SF significantly reduces methane emission from ruminants and increases VFA production without affecting the total tract digestion when compared to TMR.-
dc.description.abstractLimited researches are available on effect of TMR (Total Mixed Ration) and SF (Separate feeding) system on ruminant methane production. Two experiments were performed at two different levels of daily feed intake (2.4 and 2.0 % of BW) to investigate methane production from the two different feeding systems by using a triplicated 2 × 2 Latin square design. Each experiment was conducted using six male Holsteins with BW ranging from 230–570 kg. Animals were provided either SF or TMR containing 73% concentrate and 27% forage, with the same ratio of same ingredients and grasses, twice a day. No differences (P > 0.1) in methane emissions were observed in animals fed diets at 2.4% of BW in experiment 1. Animals fed SF at 2% BW in experiment 2 emitted significantly more methane (114.5 vs. 138.5 L/d-
dc.description.abstractP < 0.005) and lost more methane energy (3.39 vs. 4.08%-
dc.description.tableofcontents1. Introduction 1
1.1 Study background 1
1.2 Aims of research 2
2. Literature review 3
2.1. Impact of ruminal methane on environment and animal productivity 3
2.2. Dietary strategies to mitigate ruminal methane emission 5
2.2.1. Feeding level, passage rate and digestibility 5
2.2.2. Inclusion of concentrate 6
2.2.3. Particle size distribution 6
2.2.4. TMR vs separate feeding of concentrate and forage 8
2.3. Understanding rumen microbiology 10
3. Materials and Methods 15
3.1. Animal and experimental design 15
3.2. Experimental diet and feeding 15
3.3. Methane emission measurement 18
3.4. Digestion trial and rumen sampling 19
3.5. Feed particle size measurement 19
3.6. Chemical analyses 20
3.7. Genomic DNA extraction and Quantification 21
3.8. PCR amplification and library construction 21
3.9. Microbial community diversity analysis 27
3.10. Statistical analysis 27
4. Results and discussions 28
4.1. Effect of Feeding system on methane emission 28
4.2. Effect of Feeding level on methane emission 31
4.3. Effect of Particle size of roughage on methane emission 32
4.4. Effect of feeding system on ruminal pH, VFA and NH3 34
4.5. Effect of feeding system on rumen microbial diversity 42
5. Conclusion 60
6. Bibliography 62
7. Abstract in Korean 93
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dc.formatapplication/pdf-
dc.format.extent1532076 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectmethane-
dc.subjectrumen-
dc.subjectseparate feeding-
dc.subjectTMR-
dc.subject.ddc631-
dc.titleEffects of TMR and Separate Feeding System on Ruminal Methane Production, Total Digestibility, Rumen Metabolic and Microbial Profile-
dc.typeThesis-
dc.contributor.AlternativeAuthor바라니-
dc.description.degreeMaster-
dc.citation.pages93-
dc.contributor.affiliation국제농업기술대학원 국제농업기술학과-
dc.date.awarded2017-02-
Appears in Collections:
Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Theses (Master's Degree_국제농업기술학과)
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