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The effect of fermented buckwheat on producing L-carnitine and Gamma-aminobutyric acid (GABA) enriched designer eggs

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Authors
박남현
Advisor
김도만
Major
국제농업기술대학원 국제농업기술학과
Issue Date
2017-02
Publisher
서울대학교 대학원
Keywords
BuckwheatFermentationRhizopus oligosporusL-carnitineGamma-aminobutyric acidDesigner Egg
Description
학위논문 (석사)-- 서울대학교 대학원 : 국제농업기술학과, 2017. 2. 김도만.
Abstract
The potential of fermented buckwheat as feed additives was studied to increase L-carnitine and gamma-aminobutyric acid (GABA) in designer eggs. Buckwheat contains high levels of lysine, methionine, and glutamate, which are precursors for the synthesis of L-carnitine and GABA. Rhizopus oligosporus was used for the fermentation of buckwheat to produce L-carnitine and GABA that exert positive effects like enhanced metabolism, antioxidant activities, immunity, and blood pressure control.
A novel analytical method for simultaneously detecting L-carnitine and GABA was developed using LC/MS and LC/MS/MS. The fermented buckwheat extract contained 4 and
34 fold-increased L-carnitine and GABA, respectively, than normal buckwheat. Compared to control, the fermented buckwheat extract-fed group showed enriched L-carnitine (13.6%) and GABA (8.4%) in the yolk. However, only L-carnitine was significantly different (p<0.05). Egg production (9.4%), albumen weight (2.1%), and shell weight (5.8%) were significantly increased (p<0.05). There was no significant difference in yolk weight, total cholesterol (1.9%) and triglyceride (4.9%) in the yolk was lowered (p<0.05).
Fermented buckwheat as feed additives has the potential to produce L-carnitine and GABA enriched designer eggs with enhanced nutrition and homeostasis. The L-carnitine and GABA enriched designer eggs pose significance to be utilized in superfood production and supplement industries.
Language
English
URI
https://hdl.handle.net/10371/127132
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Theses (Master's Degree_국제농업기술학과)
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