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Inhibitory effect of Lactobacillus spp. isolated from healthy Korean women on vaginal pathogens
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 고광표 | - |
dc.contributor.author | Kyeongju Lee | - |
dc.date.accessioned | 2017-07-19T03:12:23Z | - |
dc.date.available | 2017-07-19T03:12:23Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000140643 | - |
dc.identifier.uri | https://hdl.handle.net/10371/128264 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 환경보건학과, 2017. 2. 고광표. | - |
dc.description.abstract | Lactobacilli play an important role in vaginal health through production of byproducts such as lactic acid, hydrogen peroxide (H2O2) and bacteroicins. The prevalence of vaginal pathogens as well as the depletion of lactobacilli are both associated with numerous adverse health outcomes including bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). Among the BV candidate bacterial species, Gardnerella vaginalis, a well-known vaginal pathogen, and Sneathia species are recently emerging as opportunistic pathogens associated with a variety of conditions associated with vaginal infections such as preterm labor, spontaneous abortion, and HPV infection. In this study, we investigated the antimicrobial activities of the supernatant of 51 Lactobacillus strains isolated from 3 healthy subjects on Gardnerella vaginalis, Sneathia sanguinegens (BV pathogens) and Candida albicans (VVC pathogen). Also, we assessed the association between the activities and levels of supernatant pH, D- and L-lactate, and production of hydrogen peroxide. As a results, the tested Lactobacillus isolates were clustered by inhibition patterns against BV and VVC pathogens into four clusters (Cluster Ⅰ~Ⅳ). Lactobacillus isolates belonging to Cluster Ⅳ showed the greatest inhibitory activities to all tested pathogens and significantly higher levels of D-/L-lactate ratio than the other clusters (P <0.0001). To clarify the association between production of lactic acid isomers and pathogen inhibitory effect, we conducted correlation analysis. D-lactate showed significant negative association with the growth rate of all of vaginal pathogens, but no vaginal pathogens associated with L-lactate. Also, to identify the origin specificity of Lactobacillus in terms of lactate isomer producing, we compared the lactate producing patterns of vagina- and gut-originated strains. As a result, we identified that D-lactate was produced by lactobacilli in a species- and origin-specific manner and that D-lactate determines inhibitory patterns of human vaginal lactobacilli isolates on different vaginal pathogens. | - |
dc.description.tableofcontents | I. Introduction 1
II. Materials and Methods 4 1. Lactobacillus strains and culture 4 2. Vaginal pathogens and culture 5 3. Characterization of Lactobacillus spp. by pH and the production of hydrogen peroxide 6 4. Characterization of Lactobacillus spp. by the production of lactate 6 5. Test of Lactobacillus spp. for bile acid tolerance 7 6. Test of Lactobacillus spp. for acid tolerance 7 7. Test of Lactobacillus spp. for antimicrobial activity against vaginal pathogens 8 8. Characterization of substrate metabolizing capacity of Lactobacillus spp. 9 9. Statistical analysis 10 III. Results 11 1. Profile of vaginal Lactobacillus strains 11 2. Acidity and hydrogen peroxide productivity of Lactobacillus spp. isolated from the vagina 15 3. Lactate productivity of Lactobacillus spp. isolated from the vagina 17 4. Bile acid tolerance of Lactobacillus spp. isolated from the vagina 19 5. Antimicrobial activity of Lactobacillus spp. isolated from the vagina on vaginal pathogens 22 6. The comparison between antimicrobial activity and the capacity to produce lactate isomers 24 7. The correlation between antimicrobial activity and the characteristics of Lactobacillus spp. isolated from the vagina 27 8. Antimicrobial activity of lactic acid on vaginal pathogens 29 9. Lactate productivity of Lactobacillus spp. by their origins 31 10. Carbohydrate fermentation profile of Lactobacillus spp. by their origins 35 IV. Discussion 37 V. Reference 43 VI. Supporting information 47 VII. 국문초록 52 | - |
dc.format | application/pdf | - |
dc.format.extent | 1911516 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Lactobacillus | - |
dc.subject | Bacterial vaginosis | - |
dc.subject | Gardenrella vaginalis | - |
dc.subject | Sneathia sanguinegens | - |
dc.subject | Candida albians | - |
dc.subject | Vaginal infection | - |
dc.subject.ddc | 363 | - |
dc.title | Inhibitory effect of Lactobacillus spp. isolated from healthy Korean women on vaginal pathogens | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 54 | - |
dc.contributor.affiliation | 보건대학원 환경보건학과 | - |
dc.date.awarded | 2017-02 | - |
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