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Riboflavin induced photo-cross-linking of collagen hydrogel and its application in tissue engineering : 라이보플라빈을 통한 콜라겐 하이드로겔의 광가교결합 및 조직공학에의 응용

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Authors

허지승

Advisor
Nathaniel S. Hwang
Major
공과대학 화학생물공학부
Issue Date
2015-02
Publisher
서울대학교 대학원
Keywords
hydrogel
Description
학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2015. 2. Nathaniel S. Hwang.
Abstract
A meniscus tear is a common knee injury but its regeneration remains a clinical challenge. Recently, collagen-based scaffolds have been applied in meniscus tissue engineering. Despite its prevalence, application of natural collagen scaffold in clinical setting is limited due to its extremely low stiffness and rapid degradation. The purpose of the present study was to increase the mechanical properties and delay degradation rate of collagen-based scaffold by photo-crosslinking using riboflavin and UV exposure. Riboflavin is a biocompatible vitamin B2 that showed minimal cytotoxicity compared to conventionally utilized photo-initiator. Furthermore, collagen photo-cross-linking with riboflavin improved mechanical properties and delayed enzymatic degradation rate of collagen scaffolds. Furthermore, in order to observe the meniscus tissue engineering, fibrochondrocytes were encapsulated. Photo-crosslinking of collagen resulted in reduced contractility and enhanced gene expression levels for the collagen II and aggrecan. Additionally, for activation of meniscus regeneration, hyaluronic acid (HA) was incorporated with collagen gel and showed increase of meniscus related gene level for the type I collagen, type II collagen, and aggrecan. Based upon these results, we demonstrate that photo-cross-linked collagen HA have potential for utilization in the scaffold-based meniscus tissue engineering.
Language
English
URI
https://hdl.handle.net/10371/129370
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