Effects of nanostructured surfaces on in vitro differentiation of multi-potent pulp cells derived from human supernumerary tooth

Abstract

Human dental pulp stem cells (hDPSCs) are multipotentmesenchymal stem cells that have several differentiation potentials. The tissue differentiated from these cells can provide insights for regenerative therapeutics and tissue engineering. The mesiodens is the most frequent form in the supernumerary tooth and dental pulp stem cells from mesiodens can be differentiated into several lineages similar to that from normal deciduous tooth. Recently, it has shown that the influence of nanostructured substrate to differentiation was dramatic positively. In this study, we examined the osteogenic and adipogenic differentiation of dental pulp cells from mesiodens,which contains hDPSCs, cultured on nanostructured substrate in the presence of each induction media. The enhancement of osteogenic and adipogenicdifferentiation on nanostructured substratewas discovered. In particular, the contribution of nanostructured substrate to the elevation of adipogenic differentiation induction was remarkable. In conclusion, specific surface nanotopography can enhance adipogenic and osteogenic differentiation in vitro, and it may contribute the development of hard tissue regeneration technology.