The effect of Human Umbilical Cord Blood-derived Mesenchymal Stem Cells in Collagenase induced Intracerebral Hemorrhage Rat model

Abstract

Purpose: Intracerebral hemorrhage (ICH) is one of the devastating types of stroke and has a high risk of morbidity and mortality. Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have potential to help the brain damage recovered following ICH. The purpose of this study is to identify beneficial effects of the transplantation of hUCB-MSCs in the ICH rat model and investigate whether hUCB-MSCs may have the ani-inflammatory properties by neurotrophic factors or cytokines for ICH brain.

Material & Method: hUCB-MSCs were transplanted in collagenase induced ICH rat model. At 2, 9, 16, 30 days after ICH, rotarod test and limb placement test were performed to measure behavioral outcomes. ICH rats were sacrificed to evaluate volume of lesion using H&E staining. Neurogenesis, angiogenesis, anti-apoptosis in the brain tissue of the rats was examined by immunofluorescence staining at 4 weeks after transplantation. Anti-inflammatory factors [TNF-, COX-2, microglia and neutrophil were analyzed by immunofluorescence staining, RT-PCR and Western blot at 3 days after transplantation. Results: hUCB-MSCs transplantation after ICH was associated with the effect of neurological benefits and reducing volume of lesion. hUBC-MSCs treated group revealed high level of neurogenesis, angiogenesis and anti-apoptosis at 4 weeks after transplantation. The expression of inflammatory factors were decreased in rats of hUCB-MSCs treated group compared with rats of control group.

Conclusion: Our study suggests that hUCB-MSCs may improve neurological outcome and modulate inflammation-associated immune cells and cytokines in ICH-induced inflammatory responses.