S-Space College of Natural Sciences (자연과학대학) Biophysics and Chemical Biology (생물물리 및 화학생물학과) Theses (Master's Degree_생물물리 및 화학생물학과)
Effect of gut microbiota on the development of atopic dermatitis in NC/Nga mice
장내 미생물이 NC/Nga 의 아토피성 피부염에 미치는 영향
- 자연과학대학 생물물리 및 화학생물학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 생물물리 및 화학생물학과, 2014. 2. 석영재.
- Recent researches on gut microbiome provided better understanding of relationship between gut microbiota and host pathology, including atopic dermatitis, of which occurrence and development are known to be associated with gut microbiota but not characterized so far. To identify intestinal microorganisms related with atopic dermatitis, specific antibiotic cocktails were developed to change the two major phylotypes of gut microbiota, of which composition was examined by pyrosequencing using 16S rRNA gene tags. NC/Nga mice, model animal for atopic dermatitis, were administered two different antibiotic cocktails targeting either Gram negative (GNA) or Gram positive (GPA) bacteria by either ad libitum drinking water or oral gavage for 6 weeks.
The GPA group showed an increased serum IgE level accompanied with more severe skin damage compared to the control and GNA groups. Bacterial community analysis data also revealed dramatic changes in the gut microbiota composition of the GPA group with a significant increase in abundance of the species Enterobacter cowanii and Enterobacter hormaechei belonging to the Proteobacteria. The involvement of the two bacteria in the pathology of atopic dermatitis was tested by oral administration to NC/Nga mice. Only the mouse group colonized with Enterobacter hormaechei showed an aggravation of skin damage and an increased serum IgE level. Our data suggest that enterobacteria, Enterobacter hormaechei, may play an important role in development and severity of atopic dermatitis in NC/Nga mice