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Predictable communities of soil bacteria in relation to nutrient concentration and successional stage, in a laboratory culture experiment. : 토양 박테리아 군집 조성에 관한 연구: 배양 환경에서의 영양분 농도와 시간에 따른 접근

DC Field Value Language
dc.contributor.advisorJonathan Adams-
dc.contributor.author송우진-
dc.date.accessioned2017-07-19T09:07:38Z-
dc.date.available2017-07-19T09:07:38Z-
dc.date.issued2015-02-
dc.identifier.other000000025151-
dc.identifier.urihttps://hdl.handle.net/10371/131583-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 생명과학부, 2015. 2. Jonathan Adams.-
dc.description.abstractThe best hope of understanding the processes that govern soil bacterial communities is to represent them as simpler microcosms, which may give hints to the ecology of natural systems. We set up a microcosm culturing experiment with soil bacteria, at a range of nutrient concentrations, and compared these over time. DNA from each replicate was analyzed using HiSeq2000 Illumina sequencing of the 16S rRNA gene. We found that each nutrient treatment, and each time point during the experiment, produces characteristic bacterial communities that occur predictably between replicates. It is clear that within the context of this experiment, many soil bacteria have distinct niches from one another, in terms of both nutrient concentration, and successional time point since a resource first became available. This fine niche differentiation may in part help to explain the coexistence of a diversity of bacteria in soils. In this experiment, we show that the unimodal relationship between nutrient concentration/time and species diversity often reported in communities of larger organisms, is also evident in microbial communities.-
dc.description.tableofcontentsTable of Contents
ABSTRACT……………………………………………………………….ⅰ
TABLE OF CONTENTS ………………………………………………ⅲ
LIST OF FIGURES................................................................ⅳ
LIST OF TABLES………………………………………………………….ⅴ
1. INTRODUCTION ………………………………………………………..1
2. METERIALS AND METHODS ………………………………………...7
2.1. Sampling, Experimental Design and DNA Extraction ………………..7
2.2. HiSeq2000 paired-end sequencing and data analysis …………………9
2.3. Statistical Analysis …………………………………………………..10
3. RESULTS ………………………………………………………………12
4. DISCUSSION ………………………………………………….………...43
4.1 Distinct bacterial community composition at each incubation sampling time point and nutrient concentration........................43
4.2. Unimodal curve in bacterial diversity along the nutrient gradient…………………………………………………….…..45
4.3 Bacterial diversity peaks at same sampling time point independent of initial nutrient concentration…………………47
5. CONCLUSIONS…………………………………………………………49
REFERENCES …………………………………………..………………...51
국문초록(Abstract in Korean)………………………………………....61
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dc.formatapplication/pdf-
dc.format.extent1780564 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectBacteria-
dc.subjectSuccession-
dc.subjectMicrobial communities-
dc.subjectMicrobial ecology-
dc.subjectNiche differentiation-
dc.subject.ddc570-
dc.titlePredictable communities of soil bacteria in relation to nutrient concentration and successional stage, in a laboratory culture experiment.-
dc.title.alternative토양 박테리아 군집 조성에 관한 연구: 배양 환경에서의 영양분 농도와 시간에 따른 접근-
dc.typeThesis-
dc.contributor.AlternativeAuthorWoojin Song-
dc.description.degreeMaster-
dc.citation.pagesⅴ, 62-
dc.contributor.affiliation자연과학대학 생명과학부-
dc.date.awarded2015-02-
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