Effect of glucose in the growth of Dictyostelium discoideum

Abstract

The glycation of proteins by glucose has been linked to the development of diabetic complications and other diseases. Early glycation is thought to involve the reaction of glucose with N- terminal chain amino groups to form Schiffs base and fructosamine adducts. The formation of the α-oxoaldehydes, glyoxal, methylglyoxal, in early glycation was investigated. Glucose degraded slowly to form glyoxal, methylglyoxal. Cells were cultured with high concentration of glucose in HL5 media showed cell growth inhibition and increase of the cell size and weight. And concentration of intracellular methylglyoxal measured drastically increased during cultured with high-dose glucose in media. This results supporting the high-dose glucose induced accumulation of methylglyoxal in vivo. To confirm this hypothesis, putrescine added under the same incubate conditions, and the result exhibited putrescine blocks cell growth inhibition caused by accumulation of methylglyoxal. These results suggest a possibility of accumulation of methylglyoxal from high-dose glucose.