Browse

이차 수정체 섬유세포 분화 과정에서 Sorting nexin 5의 역할
Role of Snx5 in differentiation of secondary lens fiber cells

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
신수빈
Advisor
공영윤
Major
자연과학대학 생명과학부
Issue Date
2015-02
Publisher
서울대학교 대학원
Keywords
Sorting nexin 5Snx5CataractLensSecondary lens fiber cells
Description
학위논문 (석사)-- 서울대학교 대학원 : 생명과학부, 2015. 2. 공영윤.
Abstract
Sorting nexin 5 (Snx5) has been known as a member of retromer complex, required for recycling of proteins back to cell membrane through Trans-Golgi-Network. Snx5 has a PX (Phospoinositide-binding structural) domain in its N-terminal and a BAR (Bin, Amphiphysin, Rvs) domain in its C-terminal. In general, Snx5 binds to phosphatidylinositol-3-monophosphate (PtdIns3P) with its PX domain and makes curvature of membrane with BAR domain. Through this interaction, Snx5 helps a separation of vesicles from endosomal compartment to pass through trans-golgi-network (TGN).
In lens development, reciprocal interactions between ligands and receptors play crucial roles. Indeed, several receptor-mediated events affect each process of lens development. Growth factors signalings are typical examples, such as bone morphogenetic protein (BMP), basic fibroblast growth factor (bFgf), and epidermal growth factor (Egf). However, it is largely unknown how those receptor levels are regulated.
Here, I show that the ablation of Snx5, a member of retromer complex, induces a cataract formation in lens of Snx5-/- mice. Histological analysis revealed that Snx5-/- mice have defective equatorial region of lens and abnormal differentiating secondary lens fiber. Also, the depletion of Snx5 resulted in overexpression of α-smooth muscle actin (α-SMA) in equatorial region of lens. Taken together, I propose that Snx5 gene plays an important role in trans-differentiation of lens fiber cells and cataract formation.
Language
English
URI
https://hdl.handle.net/10371/131585
Files in This Item:
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Master's Degree_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse