Metagenomic analysis of Eukaryote diversity in coastal areas of South Korea revealed by Illumina Miseq

Abstract

Metagenomic analysis was carried out on the multiple taxa of eukaryotes, for the first time in Korea. Each samples were obtained from fourteen sites of Jeju island and the seas surrounding the Korean peninsula (East Sea, East China Sea, Yellow Sea) during August 2015. To more focus on Metazoa, total fourteen samples were washed through with a 63㎛ mesh sieve, then amplified based on 18S rDNA V4 hypervariable region. High-throughput sequencing of PCR amplicons was performed using the Illumina MiSeq. Paired-ends sequences, ranged from 25,637-38,463 reads per sample, revealed a mean of unique 230 OTUs (operational taxonomic units, 97% similarity). PoHang, had the highest number of unique OTUs, which showed 334 OTUs, while GoChang had the lowest unique OTUs, which had 49 OTUs. A total of 319 OTUs were identified into the species level by BLASTagainst SILVA database. For Metazoa, 103 species of 101 genera belonging to 75 families in 10 phyla were represented. Among these phyla, Annelida, Arthropoda, Mollusca, Nematoda and Platyhelminthes were dominated, account for 92.23% of the total. Of these, several undescribed taxa were detected at family-level identification

Nematoda 100% (6 families), Annelida 31.6% (6 families), Arthropoda 30.8% (4 families), Mollusca 20% (4 families), Platyhelminthes 100% (10 families) compared with integrated three DBs (MABIK, KOMBIS, MRBR). Also, relatedness community structure of eukaryotes and habitat were analyzed by beta-diversity using PCoA (Principle Coordinate Analysis) plot and UPGMA (Unweighted Pair Group Method with Arithmetic mean) tree. As a result, composition of eukaryotes community is likely to be largely affected by the habitat, however, the same habitat not always guarantee the same community structure.