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The effect of ascorbic acid deficiency on bone formation in Gulo knock out mice : Gulo 유전자 적중 생쥐에서의 아스코르빈산 결핍이 골형성에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이왕재 | - |
dc.contributor.author | 김원 | - |
dc.date.accessioned | 2017-07-19T10:17:31Z | - |
dc.date.available | 2017-07-19T10:17:31Z | - |
dc.date.issued | 2012-08 | - |
dc.identifier.other | 000000005429 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132517 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2012. 8. 이왕재. | - |
dc.description.abstract | L-gulono-γ-lactone oxidase (Gulo) is an essential enzyme in the synthesis of ascorbic acid from glucose. It is known that gulo is mutated in humans and certain primates. Gulo (-/-) mice are a useful animal model of human scurvy when they are raised under an ascorbic acid-deficient condition, since Gulo (-/-) mice have a defect in the expression of the gulo gene. Based on previous findings on bone abnormalities, including multiple chondrocostal junction fractures in Gulo (-/-) mice upon ascorbic acid insufficiency, the specific factors at work are investigated on the present study. At 4 weeks after ascorbic acid withdrawal, a definite loss of weight was found in ascorbic acid-insufficient Gulo (-/-) mice. Interestingly, the plasma level of osteocalcin, which is secreted solely by osteoblasts and is thought to play a role in the metabolic regulation of bone, was dramatically decreased in ascorbic acid-insufficient Gulo (-/-) mice 3 weeks after ascorbic acid withdrawal. In addition, it was no longer detected at 4 weeks after ascorbic acid withdrawal. The tibia weight of the ascorbic acid-sufficient Gulo (-/-) mice was significantly higher than the other three groups. The trabecular bone volume was decreased near the growth plate. Moreover, we found a significant decrease in the trabecular bone attachment to the growth plate in ascorbic acid-insufficient Gulo (-/-) mice at 3 or 4 weeks after ascorbic acid withdrawal. Taken together, there are severe defects in normal bone formation as the result of ascorbic acid insufficiency, and this effect is closely related to a decrease in the osteocalcin level. | - |
dc.description.tableofcontents | Abstract i
Contents iii List of Figures iv List of Abbreviations v Introduction 1 Material and Methods 3 Results 6 Discussion 15 Reference 20 Abstract in Korean 25 | - |
dc.format | application/pdf | - |
dc.format.extent | 1444730 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | ascorbic acid | - |
dc.subject | vitamin C | - |
dc.subject | Gulo | - |
dc.subject | osteocalcin | - |
dc.subject | bone formation | - |
dc.title | The effect of ascorbic acid deficiency on bone formation in Gulo knock out mice | - |
dc.title.alternative | Gulo 유전자 적중 생쥐에서의 아스코르빈산 결핍이 골형성에 미치는 영향 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | v, 26 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2012-08 | - |
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