S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Master's Degree_의학과)
High cholesterol diet induces inflammatory cytokine expression in zebrafish
제브라피쉬에서 고콜레스테롤 식이에 의해 유도되는 염증성 싸이토카인 발현 연구
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- Leukocyte; Atherolsclerosis; Cholesterol; Inflammation; Interleukin-1β; Zebra; High cholesterol diet
- 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2014. 2. 석승혁.
- Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regarding inflammatory cytokine expression during the lipid accumulation in zebrafish larva and adult fish. In this study, the expression levels of IL-1 and TNF-α were demonstrated in high cholesterol diet (HCD)-fed zebrafish larvae, and although HCD induced vascular lipid accumulation, the cytokine expressions in the larvae were not changed by HCD. Furthermore, there was no significant difference in leukocyte accumulation in vessels between control and HCD fed group. But prolonged HCD induced IL-1 expression in spleen and liver compared to those of control zebrafish, and produced very early stage of fatty streak lesion in dorsal aorta of 19 week HCD-fed zebrafish. These results indicate that HCD induced hypercholesterolemia and atherosclerotic changes in zebrafish are very early stage, and suggest the necessity of the generation of mutant zebrafish having a disruption in a lipid metabolism-related gene leading to severe hypercholesterolemia and advanced atherosclerosis.