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The effect of interleukin (IL)-22 on human keratinocyte cell line irradiated by UVB
DC Field | Value | Language |
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dc.contributor.advisor | 이왕재 | - |
dc.contributor.author | 김예진 | - |
dc.date.accessioned | 2017-07-19T10:23:22Z | - |
dc.date.available | 2017-07-19T10:23:22Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000017073 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132644 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2014. 2. 이왕재. | - |
dc.description.abstract | Interleukin (IL)-22, a member of IL-10 family, is a potent mediator of inflammatory responses. It is produced by activated CD4+ T cells and natural killer (NK) cells, but IL-22Rα expression is restricted to nonhematopoietic cells in the skin, pancreas, intestine, liver, lung and kidney. It has recently been reported that IL-22 plays a critical role in the maintenance of epidermal homeostasis by controlling cell cycle of keratinocytes. In addition, it is already known that ultraviolet B (UVB) radiation induces skin inflammation. However, there are no reports regarding the role of UVB on the production of IL-22 and its receptor expression. Therefore, I investigated the role of IL-22 on the proliferation of UVB-irradiated human keratinocyte cell line, HaCaT and the induction of skin inflammation in terms of IL-22Rα expression on HaCaT. The expression of IL-22Rα mRNA and its protein in HaCaT was increased by UVB (100 J/m2) irradiation. Interestingly, the translocation of IL-22Rα from cytosol to membrane was increased by UVB irradiation. It is generally known that UVB suppresses the proliferation of HaCaT, but the suppressed proliferation of UVB-irradiated HaCaT was recovered by the treatment of recombinant IL-22 and culture supernatant of activated PBMCs. Finally, the production of pro-inflammatory cytokines, such as IL-1α, IL-6 and IL-18, was increased from UVB-irradiated HaCaT by the treatment of rIL-22. Taken together, IL-22 increases skin inflammation and the proliferation of HaCaT through the interaction with up-regulated IL-22Rα on HaCaT by UVB irradiation. | - |
dc.description.tableofcontents | CONTENTS
Abstract …………………………………………………ⅰ Contents …………………………………………………ⅲ List of figures ……………………………………………ⅵ List of abbreviations ……………………………………ⅷ Introduction ……………………………………………… 1 Materials and Methods 1. Cell culture …………………………………………………………5 2. UVB irradiation ……………………………………………………5 3. Measurement of cell viability and % of cell growth rate by UVB …5 4. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) ……6 5. Confocal microscopy ………………………………………………7 6. Cell proliferation assay ……………………………………………7 7. Western blot ………………………………………………………8 8. Isolation of Peripheral Blood Mononuclear Cells (PBMCs) ……… 9 9. IL-22 bioassay……………………………………………………10 10. Examination of signaling pathways for IL-22Rα expression……10 11. Enzyme-Linked Immunosorbent Assay (ELISA)…………………11 12. Statistical analysis …………………………………………………11 Results 1. UVB irradiation suppresses the growth of human keratinocyte cell line, HaCaT in a dose-dependent manner ……………………………………………………………………12 2. UVB irradiation increases the expression of IL-22Rα in human keratinocyte cell line, HaCaT ……………………………………………………………………14 3. UVB irradiation activates PI3K/Akt, but it is not involved in the regulation of the IL-22Rα expression in human keratinocyte cell line, HaCaT ……………………………………………………………………17 4. UVB induces the translocation of IL-22Rα from cytosol to membrane of human keratinocyte cell line, HaCaT ……………………………………………………………………21 5. IL-22 increases the suppressed proliferation of UVB-irradiated HaCaT ……………………………………………………………………23 6. IL-22 increases the production of IL-1α, IL-6 and IL-18 in UVB-irradiated HaCaT ……………………………………………………………………28 Discussion ………………………………………………30 References ………………………………………………36 Abstract in Korean ………………………………………46 | - |
dc.format | application/pdf | - |
dc.format.extent | 1112547 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | inflammation | - |
dc.subject | proliferation | - |
dc.subject | HaCaT | - |
dc.subject | IL-22 | - |
dc.subject | UVB | - |
dc.subject.ddc | 610 | - |
dc.title | The effect of interleukin (IL)-22 on human keratinocyte cell line irradiated by UVB | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Kim Yejin | - |
dc.description.degree | Master | - |
dc.citation.pages | Viii, 47 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2014-02 | - |
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