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Probiotic suppression of the H. pylori-induced responses by conjugated linoleic acids in a gastric epithelial cell line : 포합 리놀레산에 의한 위상피세포주 내 헬리코박터균 반응의 억제
DC Field | Value | Language |
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dc.contributor.advisor | 정현채 | - |
dc.contributor.author | 황성욱 | - |
dc.date.accessioned | 2017-07-19T10:23:45Z | - |
dc.date.available | 2017-07-19T10:23:45Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000017339 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132653 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2014. 2. 정현채. | - |
dc.description.abstract | Introduction: Conjugated linoleic acids (CLA) produced by Lactobacillus acidophilus was reported to decrease the activation of nuclear factor-kappa B. CLA was suggested as one of the anti-inflammatory molecular mechanisms of probiotics. In the present study, the effects of CLA on H. pylori-induced multiple responses were evaluated.
Methods: IL-8, TNF-α and iNOS were measured in mRNA and/or protein levels in AGS cells after pretreatment with CLA or CLA-containing conditioned medium (CM) produced by Lactobacillus acidophilus or Lactobacillus plantarum. Results: The increased expressions of IL-8 mRNA/protein and TNF-α mRNA were significantly suppressed by pretreatment with CM or CLA. The levels of IL-8 protein and TNF-α mRNA were suppressed by CM pretreatment better than CLA. The expression of iNOS mRNA was also significantly inhibited by CM pretreatment. Conclusions: These results suggest that the suppression of multiple mediators by CLA-containing CM plays a key role in the anti-inflammatory and anti-carcinogenic effects of probiotics on H. pylori infection. | - |
dc.description.tableofcontents | Introduction
Materials and Methods Cell culture H. pylori strain Preparation of CM, measurement of CLA contained in CM and cell treatment RNA extraction, reverse transcription and real-time PCR Measurement of IL-8 and TNF-α protein by enzyme-linked immunosorbent assay Statistical analyses Results The amounts of CLA in culture supernatants The effect of CLA on IL-8 mRNA expression in AGS cells infected with H. pylori The effect of CM on IL-8 mRNA expression in AGS cells infected with H. pylori The effects of CLA and CM on IL-8 protein production in AGS cells infected with H. pylori The effect of CLA on mRNA expression and protein production of TNF-α in the H. pylori-infected AGS cells IL-8 and TNF-α mRNA expressions after pretreatment with medium not containing CLA in the H. pylori-infected AGS cells Effect of CLA on iNOS mRNA expression in the H. pylori-infected AGS cells Discussion References Abstract in Korean | - |
dc.format | application/pdf | - |
dc.format.extent | 1314837 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Helicobacter pylori | - |
dc.subject | Probiotics | - |
dc.subject | Conjugated linoleic acids | - |
dc.subject | Interleukin-8 | - |
dc.subject | Tumor necrosis factor-alpha | - |
dc.subject | Inducible nitric oxide synthase | - |
dc.subject.ddc | 610 | - |
dc.title | Probiotic suppression of the H. pylori-induced responses by conjugated linoleic acids in a gastric epithelial cell line | - |
dc.title.alternative | 포합 리놀레산에 의한 위상피세포주 내 헬리코박터균 반응의 억제 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Sung Wook Hwang | - |
dc.description.degree | Master | - |
dc.citation.pages | vi, 33 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2014-02 | - |
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