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Investigation of the anti-inflammatory effect of GV1001, a peptide derived from human telomerase reverse transcriptase (hTERT) sequence : 사람 Telomerase 역전사효소 서열 유래 펩타이드 GV1001의 항염 효능에 대한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강재승 | - |
dc.contributor.author | 최지예 | - |
dc.date.accessioned | 2017-07-19T10:30:32Z | - |
dc.date.available | 2017-07-19T10:30:32Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.other | 000000067125 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132794 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의학과(해부학 전공), 2015. 8. 강재승. | - |
dc.description.abstract | GV1001 is a peptide derived from human telomerase reverse transcriptase (hTERT) sequence, and has anti-cancer and anti-inflammatory effect. Enolase1 (ENO1) is a glycolytic enzyme and its stimulation induces to produce a large amount of pro-inflammatory cytokines from concanavalin (Con) A -activated peripheral blood mononuclear cells (PBMCs) and from ENO1 expressing monocytes and macrophages from rheumatoid arthritis (RA) patients. However, it is still unknown whether GV1001 could regulate the ENO1-mediated pro-inflammatory cytokines production. Therefore, I investigated whether GV1001 regulates ENO1-mediated pro-inflammatory cytokines production as an anti-inflammatory peptide. First, I found that GV1001 does not affect the expression of ENO1 on Con A-activated PBMCs and RA PBMCs. However, ENO1 stimulation increased the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) from Con A-activated PBMCs. And it is down-regulated by the pre-treatment of GV1001. GV1001 also decreases the production of pro-inflammatory cytokines from ENO1 stimulated RA PBMCs. And then I examined what kinds of signaling molecules are involved in the down-regulation of ENO1-mediated pro-inflammatory cytokine production by GV1001. When ENO1 on the surface of Con A-activated PBMCs and RA PBMCs is stimulated, I found that p38 MAPK and NF-κB are activated. However, these are successfully suppressed by the pre-treatment of GV1001. Taken together, GV1001 might be a useful anti-inflammatory peptide via the down-regulation of pro-inflammatory cytokine production and the suppression of the p38 MAPK and NF-κB activation by ENO1 stimulation. | - |
dc.description.tableofcontents | CONTENT
Abstract ............................................................... ⅰ Contents .............................................................. ⅳ List of Figures ................................................... ⅶ List of Abbreviations ........................................ ⅹ Introduction ......................................................... 1 Materials and Methods 1. Isolation of PBMCs .................................................................... 5 2. Stimulation of PBMCs with Con A .......................................... 5 3. Preparation of Anti-ENO1 monoclonal antibody.................... 6 4. ENO1 stimulation ....................................................................... 7 5. GV1001 treatment ..................................................................... 7 6. Flow cytometry analysis .......................................................... 7 7. Enzyme-Linked Immunosorbent Assay (ELISA) ................. 8 8. Inhibitor study for signal pathway ........................................ 9 9. Immunoblotting ........................................................................... 9 10. Statistical analysis ................................................................. 10 Results 1. Con A stimulation increases ENO1 expression on the surface of PBMCs ................................................................................................... 12 2. GV1001 doesn't affect ENO1 expression on PBMCs by Con A treatment ................................................................................................... 16 3. GV1001 decreases the production of TNF-α, IL-1β and IL-6 by ENO1 stimulation ................................................................................................... 18 4. GV1001 suppresses the activation of p38 MAPK and NF-κB in Con-A activated PBMCs: ELISA ................................................................................................... 22 5. GV1001 suppresses the activation of p38 MAPK and NF-κB in Con-A activated PBMCs: Immunoblotting ................................................................................................... 25 6. GV1001 doesn't change the expression level of ENO1 on PBMCs from RA patients ................................................................................................... 31 7. GV1001 decreases the production of TNF-α, IL-1β and IL-6 from RA PBMCs by ENO1 stimulation ................................................................................................... 34 8. GV1001 suppresses the activation of p38 MAPK and NF-κB in RA PBMCs. ................................................................................................... 37 Discussion .......................................................... 39 References ......................................................... 44 Abstracts in Korean ......................................... 52 | - |
dc.format | application/pdf | - |
dc.format.extent | 1273024 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | inflammation | - |
dc.subject | GV1001 | - |
dc.subject | enolase1 | - |
dc.subject | rheumatoid arthritis | - |
dc.subject | p38 MAPK | - |
dc.subject | NF-κB | - |
dc.subject.ddc | 610 | - |
dc.title | Investigation of the anti-inflammatory effect of GV1001, a peptide derived from human telomerase reverse transcriptase (hTERT) sequence | - |
dc.title.alternative | 사람 Telomerase 역전사효소 서열 유래 펩타이드 GV1001의 항염 효능에 대한 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Jiyea Choi | - |
dc.description.degree | Master | - |
dc.citation.pages | 53 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2015-08 | - |
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