Analysis of the coagulation cascade and fibrinolysis system components in allergic rhinitis murine model

Abstract

Background : Dysregulation of the coagulation cascade and fibrinolysis system may play an etiologic role in many diseases. Allergic diseases such as bronchial asthma, atopic dermatitis, and conjunctivitis are also associated with fibrin accumulation caused by the change in hemostasis. Nevertheless there has been only a few studies dealing with the relation between allergic rhinitis (AR) and the coagulation system. Objective : This study was conduct to investigate the change of coagulation and fibrinolysis cascade components in allergic rhinitis murine model Methods : BALB/c mice were sensitized and challenged with ovalbumin. Multiple parameters related to coagulation cascade and fibrinolysis system such as Factor II, V, VII, X, XIII, tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor-1 (PAI-1), and fibrin were compared between the allergic rhinitis model group and the control group. Results : The symptom scores and eosinophil counts were higher in the allergic rhinitis model group than the control group (p< .01). The mRNA expression level of u-PA (p= .040), PAI-1 (p= .054), Factor II (p= .038), Factor X (p= .036) was significantly higher in the allergic rhinitis model group. Most of the fibrinolysis system and coagulation cascade components were localized at the epithelium, endothelium and submucosal glands of the nasal mucosa on immunohistochemical staining and there was a down regulation of u-PA in AR group while fibrin deposition was much prominent in the AR model group. Conclusion : In allergic rhinitis condition, the coagulation cascade was up-regulated and the fibrinolysis system was down-regulated which seemed that the change of both components lead to fibrin deposition in allergic rhinitis mouse model.