S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Master's Degree_의학과)
1,1′-Dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide-loaded poly(lactide-co-glycolide) nanoparticles increase local retention after paraspinal muscle injection
나노파티클을 이용한 척추 주위 근육 주사 후 체내 지속 시간 증가에 대한 연구
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- PLGA nanoparticle; paraspinal muscle; intramuscular injection; local retention time; nude mouse; fluorescence; IVIS imaging system
- 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2017. 2. 이준우.
- Poly(lactide-co-glycolide) (PLGA) nanoparticles are promising materials for the development of new drug releasing systems. In this study, PLGA nanoparticles were doped with the fluorescent material 1,1′-dioctadecyl-3,3,3′,3′tetramethylindotricarbocyanine iodide (DiR), and the retention of the nanoparticles was evaluated in mice. Mice (n = 20) were injected with 0.1 mL DiR-loaded PLGA nanoparticles (200 nm) into the right paraspinal muscle, and the same volume of pure DiR solution was injected into the left paraspinal muscle. Fluorescence images were obtained using an IVIS Imaging System. Fluorescent images were taken 1 day after the injection, and seven more images were taken at 1-week intervals. After 7 weeks, 12 mice showed a right-sided dominant signal, representing the DiR-loaded PLGA nanoparticles
five mice showed a left-side dominant signal, representing the free DiR solution
and three mice showed no signal at all beginning 1 day after the injection. These last three mice were excluded from further analysis owing to apparent injection failure. Notably, DiR-loaded PLGA nanoparticles showed significantly higher fluorescence intensity over the 7-week period. In conclusion, the results of the current study suggested that therapeutic agents that bind to PLGA nanoparticles may exhibit prolonged retention times.