Publications
Detailed Information
Role of Bile Acid-induced Fibroblast Growth Factor 19 Expression in Hepatocellular Carcinoma Cell Proliferation : 담즙산으로 유도된 fibroblast growth–19가 간세포암의 증식에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 윤정환 | - |
dc.contributor.author | 장영 | - |
dc.date.accessioned | 2017-07-19T10:38:22Z | - |
dc.date.available | 2017-07-19T10:38:22Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000142750 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132959 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의학과, 2017. 2. 윤정환. | - |
dc.description.abstract | Background: Fibroblast growth factor 19 (FGF19) is produced by enterocytes and
acts on FGF receptor 4 (FGFR4) to down-regulate cholesterol 7α-hydroxylase (CYP7A1), the key gene involved in bile acid synthesis. Abundant bile acid increases the production of FGF19, resulting in a negative feedback cycle for bile synthesis. We hypothesized that bile acid-induced FGF19 expression also may promote hepatocellular carcinoma (HCC) cell proliferation. Methods: Huh-BAT and SNU761 HCC cell lines were treated with control small interfering RNA (siRNA) or FGF19-specific siRNA with or without chenodeoxycholic acid (CDCA, bile acid) or obeticholic acid (OCA). Cell viability, gene expression, and protein expression were assessed by MTS cell proliferation assay, RT-qPCR, and immunoblotting. Results: HCC cells treated with 25 μM CDCA exhibited increased cell proliferation and elevated levels of FGF19 mRNA. Cell transfection with FGF19- or FGFR4-specific siRNA attenuated bile acid-induced HCC cell proliferation, and FGF19-specific siRNA reduced the phosphorylation of p42/44 mitogen-activated protein kinase. These results suggest that bile acid-induced HCC cell proliferation is mediated by autocrine FGF19 signaling. Conclusions: This study demonstrated that inhibition of FGF19/FGFR4 signaling suppresses bile acid-induced HCC cell proliferation. Therefore, suppression of FGF19 or FGFR4 expression may be a therapeutic strategy for attenuating the growth of HCC with cholestasis. | - |
dc.description.tableofcontents | Introduction 1
Materials and Methods 3 Results 7 Discussion 18 References 21 Abstract in Korean 26 | - |
dc.format | application/pdf | - |
dc.format.extent | 769662 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Fibroblast growth factor 19 | - |
dc.subject | Hepatocellular carcinoma | - |
dc.subject | Bile acid | - |
dc.subject.ddc | 610 | - |
dc.title | Role of Bile Acid-induced Fibroblast Growth Factor 19 Expression in Hepatocellular Carcinoma Cell Proliferation | - |
dc.title.alternative | 담즙산으로 유도된 fibroblast growth–19가 간세포암의 증식에 미치는 영향 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 27 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2017-02 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.