Comparative Analysis of the Aqueous Humor Between Exudative Age-related Macular Degeneration Patients and Control Group

Abstract

Purpose: To compare the density of more than 500 molecules in aqueous humor between exudative age-related macular degeneration (AMD) patients and control group. Methods: Aqueous humors were acquired from 18 patients with exudative AMD and 15 control group before they were mixed in each group. Raybio® human antibody array technology to screen 507 molecules simultaneously was used to find out any difference of these molecules in densities between 2 groups. Results: Twenty molecules in aqueous humor were measured in different densities between 2 groups. Twelve molecules including insulin-like growth factor binding protein 2 (IGFBP-2), IGFBP-6, IGFBP-7, and glucocorticoid induced tumor necrosis factor receptor family related gene (GITR) ligand were detected in high densities in exudative AMD patients and 8 molecules including C-C motif chemokine receptor 7 (CCR7), CCR8, CCR9, and eotaxin-3 were detected in high densities in control group. Conclusion: We found out that IGFBPs and GITR ligand were measured in high levels in aqueous humor of eyes with exudative AMD in comparison of control group using Raybio® antibody array.