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Enhanced Lipopolysaccharide Induced Monocyte Activation by Recombinant Human Soluble Cluster of Differentiation 14 : 사람 유래 수용성 CD14 재조합 단백질에 의해 증가된 LPS유도 단핵구 활성화
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박정규 | - |
dc.contributor.author | 강성준 | - |
dc.date.accessioned | 2017-07-19T10:40:32Z | - |
dc.date.available | 2017-07-19T10:40:32Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.other | 000000022248 | - |
dc.identifier.uri | https://hdl.handle.net/10371/132999 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의과학과, 2014. 8. 박정규. | - |
dc.description.abstract | Introduction: CD14 is the first described pattern recognition receptors that detect LPS. It has two form, one is membrane-bound form and the other is soluble form. Over the past few decades, a considerable number of studies have been conducted on soluble CD14. But the exact physiological role of soluble CD14 is still controversial. In this thesis, I would like to investigate the precise physiological role of soluble CD14 on human blood monocytes activation.
Methods: To investigate the physiological role of soluble CD14, Recombinant human soluble CD14 was cloning, expression, and purified. Then, pro-inflammatory cytokine IL-6 and TNF-a secretion level from human PBMC was checked by CBA assay. After that, Phosphorylation level of NF-kB was measured by western blot from THP-1 human monocytic cell line. Results: The following results were obtained. First, soluble CD14 increased pro-inflammatory cytokine IL-6 and TNF-a secretion in human PBMC at steady state (10 pg/ml) and septic state (1ng/ml) concentration of LPS. Second, soluble CD14 augmented LPS-induced phosphorylation of NF-kappa B in THP-1 cell line. Conclusions: Taken together, these results lead to the conclusion that soluble CD14 can increase LPS-induced immune responses like pro-inflammatory cytokine secretion, and phosphorylation of NF-kappa B. Therefore, it seems to be reasonable to conclude that soluble CD14 can augment LPS-induced immune responses. | - |
dc.description.tableofcontents | Abstract i
Contents ii List of Tables and Figures iii List of Abbreviations iv Introduction 1 Material and Methods 4 Results 14 Discussion 33 References 35 Abstract in Korean 36 | - |
dc.format | application/pdf | - |
dc.format.extent | 961651 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | CD14 | - |
dc.subject | soluble CD14 | - |
dc.subject | LPS | - |
dc.subject | Phospho NF-kB | - |
dc.subject | PBMC | - |
dc.subject | Proinflammatory cytokines | - |
dc.subject | IL-6 | - |
dc.subject | TNF-a | - |
dc.subject.ddc | 610 | - |
dc.title | Enhanced Lipopolysaccharide Induced Monocyte Activation by Recombinant Human Soluble Cluster of Differentiation 14 | - |
dc.title.alternative | 사람 유래 수용성 CD14 재조합 단백질에 의해 증가된 LPS유도 단핵구 활성화 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | vi, 36 | - |
dc.contributor.affiliation | 의과대학 의과학과 | - |
dc.date.awarded | 2014-08 | - |
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