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Protective immunity induced by Respiratory Syncytial Virus Glycoprotein fragment in Neonatal Mice
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강창율 | - |
dc.contributor.author | 노유란 | - |
dc.date.accessioned | 2017-07-19T11:03:31Z | - |
dc.date.available | 2017-07-19T11:03:31Z | - |
dc.date.issued | 2012-08 | - |
dc.identifier.other | 000000003541 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133320 | - |
dc.description | 학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2012. 8. 강창율. | - |
dc.description.abstract | Respiratory syncytial virus (RSV) is the major cause of severe lower respiratory tract infection in infants and the elderly worldwide. The significant morbidity and mortality associated with this infection underscores the urgent need for an RSV vaccine development. In this study, we firstly showed that intranasal administration of RSV glycoprotein core fragment (Gcf) to neonatal mice was capable of inducing systemic humoral immune responses and had protective efficacy against RSV without lung eosinophilia, though antibody response was shifted to Th2. Next, we examined whether the presence of maternal anti-RSV antibodies affects the responsiveness and protection efficacy of Gcf in newborn mice, since infants can have RSV specific maternal antibodies due to frequent re-infection of RSV to adults. Intranasal administration of Gcf induced antibody response, IFN-γ secretion and protected mice against RSV challenge without lung eosinophilia even in the presence of high level of RSV-specific maternal antibodies. Thus, our study suggests that Gcf can be applied as an effective and safe RSV vaccine during the neonatal period. | - |
dc.description.tableofcontents | Contents
Contents Ⅰ List of Figures Ⅱ Abbreviation Ⅲ Abstract Ⅳ 1. Introduction 1 2. Materials and Methods 5 2.1 Mice 5 2.2 Preparation of antigen and RSV stocks 5 2.3 Immunization and challenge 6 2.4 Analysis of cells in BAL fluids 8 2.5 RSV detection and cytokine analysis in the lung 9 2.6 ELISA 11 2.7 Statistical analysis 13 3. Results 14 3.1 Gcf vaccination in neonatal mice induces humoral immunity 14 3.2 Effect of Gcf immunization on lung eosinophilia and protection against RSV 17 3.3 Gcf/CT vaccination induces immune response regardless of maternal antibody 20 3.4 Maternal antibodies have little effect on recruitment of eosinophil and protective efficacy against RSV challenge 24 4. Discussions 29 5. References 34 한글 초록 39 | - |
dc.format | application/pdf | - |
dc.format.extent | 468999 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Respiratory syncytial virus | - |
dc.subject | Glycoprotein | - |
dc.subject | Neonate | - |
dc.subject | Vaccine | - |
dc.subject | Maternal effect | - |
dc.subject | Mucosal | - |
dc.subject.ddc | 610 | - |
dc.title | Protective immunity induced by Respiratory Syncytial Virus Glycoprotein fragment in Neonatal Mice | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | Ⅴ, 39 | - |
dc.contributor.affiliation | 융합과학기술대학원 분자의학 및 바이오제약학과 | - |
dc.date.awarded | 2012-08 | - |
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