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Mechanism of KAI1 that regulated cell cycle in hematopoietic stem cell : 조혈모세포의 세포주기를 조절하는 KAI1 유전자의 분자기전 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김효수 | - |
| dc.contributor.author | 이환 | - |
| dc.date.accessioned | 2017-07-19T11:07:32Z | - |
| dc.date.available | 2018-03-30 | - |
| dc.date.issued | 2015-02 | - |
| dc.identifier.other | 000000026695 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/133370 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2015. 2. 김효수. | - |
| dc.description.abstract | Hematopoiesis is regulated by quiescent long term repopulating hematopoietic stem cells (LT-HSCs) in the bone marrow (BM) niche. KAI1 belongs to tetraspanin superfamily and is known as suppressor of tumor metastasis. In this study, KAI1 was expressed selectively in LT-HSCs located in the BM, but not in other stem-progenitor cells. We generated KAI1-/- mice and observed that LT-HSCs selectively decreased in number because LT-HSCs lost quiescence. KAI1 repressed cell cycle by induction of TGF-β1/Smad3 signaling via PKC, leading to induction of CDK inhibitors and cycle arrest. After ablation of BM by 5-fluorouracil treatment, recovery of cells in the BM was significantly retarded in KAI1-/- mice. Mice that received KAI1-/- HSCs showed a decrease in LT-HSCs compared with those receiving WT-HSCs. KAI1 was expressed also in human LT-HSCs, and the majority of KAI1+ LT-HSCs were quiescent. Thus, KAI1 is a previously unidentified functional surface marker of LT-HSCs and maintains LT-HSC quiescence. | - |
| dc.description.tableofcontents | CONTENTS
Abstract ·································································ⅰ Contents ································································· ⅲ List of Figures ······················································· ⅳ Introduction ··························································· 1 Materials and Methods ········································· 4 Results ··································································· 15 Discussion ····························································· 22 Figures ··································································· 25 References ···························································· 41 Abstract (Korean) ················································ 45 | - |
| dc.format | application/pdf | - |
| dc.format.extent | 1175889 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | hematopoietic stem cell | - |
| dc.subject.ddc | 610 | - |
| dc.title | Mechanism of KAI1 that regulated cell cycle in hematopoietic stem cell | - |
| dc.title.alternative | 조혈모세포의 세포주기를 조절하는 KAI1 유전자의 분자기전 연구 | - |
| dc.type | Thesis | - |
| dc.description.degree | Master | - |
| dc.citation.pages | iv, 46 | - |
| dc.contributor.affiliation | 융합과학기술대학원 분자의학 및 바이오제약학과 | - |
| dc.date.awarded | 2015-02 | - |
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