Publications
Detailed Information
Analyzing biomolecular interactions by affinity-directed mass spectrometry : 친화력 기반 질량분석법을 이용한 생체분자 상호작용 분석 연구
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Advisor
- 이유진
- Major
- 융합과학기술대학원 분자의학 및 바이오제약학과
- Issue Date
- 2015-08
- Publisher
- 서울대학교 융합과학기술대학원
- Keywords
- Affinity-directed mass spectrometry ; Biomolecular interactions ; Molecular binding simulations ; Mass spectrometric epitope mapping ; Peptide microarray analysis
- Description
- 학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2015. 8. 이유진.
- Abstract
- Protein affinity selection method utilizing mass spectrometry has now become an invaluable tool for identification of protein binding partners. Although the affinity-directed mass spectrometry analytical platform for selectively capturing interacting proteins has been implemented to discover many missing ligands, determination of true binding partners from co-purified proteins in the affinity-purified sample is extremely challenging. The challenge can be addressed by biophysical characterizations of newly identified binding partners prior to characterizing their biological associations. Several biophysical characterization methods such as (i) immunoassays (ii) molecular binding simulation (iii) mass spectrometry-based binding epitope mapping and (iv) peptide microarray-based binding epitope mapping can be implemented to evaluate the binding interaction. Herein, adapting the affinity-directed mass spectrometry method, we identified several potential ligands of a specific protein expressed on the surface of immune cells. Among them, we selected one immune cell-specific binding protein as a ligand based on its abundance. We further evaluated the binding interaction between the immune cell expressed protein and its selected ligand using the biophysical assays and confirmed that the selected ligand is specifically interacting with an immune cell specific expressed protein. The affinity-directed mass spectrometry analytical platform implemented in this investigation is expected to be of general use and to facilitate more accurate identification of many missing ligands involved in various biological processes.
- Language
- English
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.