Serum levels of IgG antibodies against alpha-enolase are increased in patients with Behçets disease and are associated with the severity of oral ulcers : 베체트병 환자에서 혈청 항 알파 에놀레이즈 항체 역가와 구강 궤양과의 연관성에 관한 연구

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융합과학기술대학원 분자의학 및 바이오제약학과
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서울대학교 융합과학기술대학원
anti-alpha-enolase antibody
학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 융합과학기술대학원 분자의학 및 바이오제약학과, 2016. 2. 송영욱.
Behcets disease (BD) is a chronic inflammatory disease of unknown etiology, characterized by recurrent oral and genital ulcers, skin lesions, uveitis, and arthritis. It is regarded as vasculitis and anti-endothelial cell antibodies (AECA) are found in patients with BD. One of the endothelial cell antibodies was reported to recognize alpha-enolase. This study aimed to investigate the association between anti-alpha-enolase antibody (AEA) and clinical manifestations or disease activity of BD. To measure serum AEA level in patients with BD, we performed Western blot analysis and ELISA assays. AEA was more frequently found in patients with BD (82.4%) than in healthy controls (66.7%) on Western blots. Serum AEA titers were increased in BD patients compared to healthy controls (1578.2 ± 115.7 vs. 1149.9 ± 134.3 AU, p = 0.037) and were correlated with the number of oral ulcers in BD patients (Spearmans coefficient = 0.275, p = 0.012). BD patients with oral ulcers were divided into mild (0~2 oral ulcers) and severe (greater than 2 oral ulcers) groups based on the cumulative number of oral ulcers in the 4 weeks prior to blood sampling. Patients in the severe group exhibited significantly higher AEA titer than did those with mild oral ulcers or healthy controls (Kruskal-Wallis test
p = 0.001). Skin lesions, uveitis, and arthritis were not associated with serum AEA levels. Cell surface alpha-enolase expression was examined using flow cytometry to investigate the correlation with serum AEA titer. Frequency of surface α-enolase-expressing cells was increased in patients with BD in several cell types, including lymphocytes and monocytes. Alpha-enolase expression in lymphocytes was correlated with serum AEA titer (Spearman's correlation coefficient = 0.417, p = 0.034). In conclusion, serum AEA titer is increased in BD patients and is associated with the number of oral ulcers. These results suggest that AEA may be useful as a serological marker of Behçets disease, particularly in patients with severe oral ulcers.
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Theses (Master's Degree_분자의학 및 바이오제약학과)
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