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Chemical Constituents of the Seeds of Aesculus turbinata and Their PEDV Inhibitory Activity : 칠엽수 열매로부터 얻은 화학성분과 PEDV 저해 활성 확인
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 오원근 | - |
dc.contributor.author | 김지원 | - |
dc.date.accessioned | 2017-07-19T11:26:18Z | - |
dc.date.available | 2017-07-19T11:26:18Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000141234 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133660 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과(생약학전공), 2017. 2. 오원근. | - |
dc.description.abstract | Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high fatality of piglets, influencing the swine industry. Japanese horse chestnut (seed of Aesculus turbinata) contains many saponin mixtures, called escins, and has been used for a long time as a traditional medicinal plant. Structure-activity relationship (SAR) studies on escins have revealed that acylations at C-21 and C-22 with angeloyl or tigoloyl groups were important for their cytotoxic effects. However, the strong cytotoxicity of escins makes them hard to utilize for other diseases and to develop as nutraceuticals. In this research, we investigated whether escin derivatives 1-7 (including new compounds 2, 3, 5 and 6), without the angeloyl or tigloyl groups and with modified glycosidic linkages by hydrolysis, have PEDV inhibitory effects with less cytotoxicity than the major compounds 8-10 isolated from the extract. Compounds 1-7 had no cytotoxicity at 20 μM on VERO cells, while compounds 8-10 showed strong cytotoxicity at similar concentrations, and also had moderate inhibitory activities on PEDV. Our results suggest that escin derivatives, which were prepared by two-step reactions, showed strong inhibitory activities on PEDV replication with less cytotoxicity. These studies proposed a method to utilize Japanese horse chestnut for treating PEDV with less cytotoxic effects, and to increase the diversity of its bioactive compounds. | - |
dc.description.tableofcontents | I. Introduction 1
1. Coronaviruses 1 2. Porcine epidemic diarrhea virus 2 3. Aesculus turbinata Blume 2 II. Materials and Methods 4 1. Plant materials 4 2. Reagents 4 3. Experimental instruments 5 4. Extraction, reaction and isolation schemes 6 5. Bioassay procedures 16 III. Results and Discussion 19 1. Structural elucidation of isolated compounds (1-10) 19 2. Cytotoxicity of fractions and isolated compounds 46 3. PEDV inhibitory activities of fractions and isolated compounds 48 IV. Conclusions 57 V. References 58 국문초록 62 | - |
dc.format | application/pdf | - |
dc.format.extent | 3787178 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Aesculus turbinata Bl. | - |
dc.subject | Sapindaceae | - |
dc.subject | escin | - |
dc.subject | cytotoxicity | - |
dc.subject | hydrolysis | - |
dc.subject | oleanane-type triterpenoids | - |
dc.subject | PEDV. | - |
dc.subject.ddc | 615 | - |
dc.title | Chemical Constituents of the Seeds of Aesculus turbinata and Their PEDV Inhibitory Activity | - |
dc.title.alternative | 칠엽수 열매로부터 얻은 화학성분과 PEDV 저해 활성 확인 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Kim Jiwon | - |
dc.description.degree | Master | - |
dc.citation.pages | 63 | - |
dc.contributor.affiliation | 약학대학 약학과 | - |
dc.date.awarded | 2017-02 | - |
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