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Characterization of a T7-like lytic bacteriophage (ɸSG-JL2) of Salmonella enterica serovar Gallinarum biovar Gallinarum
Salmonella Gallinarum에 특이적인 T7-유사 용균 박테리오파지(ΦSG-JL2)의 특성 분석

DC Field Value Language
dc.contributor.advisor김재홍-
dc.contributor.author정지혜-
dc.date.accessioned2017-07-19T11:28:22Z-
dc.date.available2017-07-19T11:28:22Z-
dc.date.issued2013-02-
dc.identifier.other000000008872-
dc.identifier.urihttps://hdl.handle.net/10371/133692-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 수의학과(수의미생물학전공), 2013. 2. 김재홍.-
dc.description.abstractɸSG-JL2 is a newly discovered lytic bacteriophage infecting Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum), but is non-lytic to a rough vaccine strain of S. Gallinarum(SG-9R), Salmonella Enteritidis, Salmonella Typhimurium, and Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum). The ɸSG-JL2 genome is 38,815 bp in length (GC content of 50.9% and 230 bp length direct terminal repeats) and 55 putative genes may be transcribed from the same strand. Functions were assigned to 30 genes based on the high amino acid similarity with known proteins. Most of the expected proteins except tail fiber (31.9%) and overall organization of genomes were similar to yersiniophage ɸYeO3-12. ɸSG-JL2 could be classified as a new T7-like virus and represents the first S. Gallinarum phage genome to be sequenced. On the basis of intraspecific ratios of nonsynonymous to synonymous nucleotide changes (Pi[a]/Pi[s]), gene 2 encoding host RNA polymerase inhibitor displayed Darwinian positive selection. Pre-treatment of chickens with ɸSG-JL2 before intratracheal challenge with wild-type S. Gallinarum protected most birds from fowl typhoid. Therefore, ɸSG-JL2 may be useful for differentiation of S. Gallinarum from other Salmonella serotypes, prophylactic application to fowl typhoid control, and understanding of vertical evolution of T7-like viruses.-
dc.description.tableofcontentsCONTENTS

ABSTRACT .......................................................... i
CONTENTS ......................................................... iii
LIST OF FIGURES ................................................. v
LIST OF TABLES .................................................. vi
1. INTRODUCTION ................................................ 1
2. MATERIALS AND METHODS .............................. 3
2.1. Bacteria, phage, and media ............................. 3
2.2. Phage isolation, cloning, and propagation .......... 3
2.3. Host range determination .................................. 4
2.4. Electron microscopy ........................................ 5
2.5. Heat and pH susceptibility ................................. 5
2.6. One-step growth curve ..................................... 5
2.7. DNA extraction, cloning, PCR, and sequencing ... 6
2.8. Sequence analysis .......................................... 7
2.9. Prophylactic efficacy of ɸSG-JL2 against fowl typhoid in chickens ........................................................... 8
2.10. Statistical analysis ......................................... 9
3. RESULTS .......................................................... 10
3.1. Host range of ɸSG-JL2 ...................................... 10
3.2. Morphology of ɸSG-JL2 ..................................... 10
3.3. One-step growth curve of ɸSG-JL2 ..................... 10
3.4. Heat and pH susceptibility of ɸSG-JL2 ................. 12
3.5. Determination of the ɸSG-JL2 genome sequence .. 12
3.6. Regulatory elements of ɸSG-JL2 ......................... 15
3.7. Origins of DNA replication .................................. 18
3.8. Genome ends of ɸSG-JL2 .................................. 21
3.9. Other features of the nucleotide sequence ........... 21
3.10. Translational features of ɸSG-JL2 ...................... 24
3.11. Identification of proteins involved in host adaptation
of ɸSG-JL2 ............................................................ 26
3.12. Prophylactic efficacy of ɸSG-JL2 against fowl typhoid
in chickens ............................................................ 28
4. DISCUSSION ...................................................... 30
REFERENCE .......................................................... 37
국문초록 ................................................................ 48
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dc.formatapplication/pdf-
dc.format.extent968656 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectT7-like virus-
dc.subjectS. Gallinarum-
dc.subjectgenome analysis-
dc.subjecthost adaptation-
dc.subjectprophylactic application-
dc.subject.ddc636-
dc.titleCharacterization of a T7-like lytic bacteriophage (ɸSG-JL2) of Salmonella enterica serovar Gallinarum biovar Gallinarum-
dc.title.alternativeSalmonella Gallinarum에 특이적인 T7-유사 용균 박테리오파지(ΦSG-JL2)의 특성 분석-
dc.typeThesis-
dc.contributor.AlternativeAuthorJihye Jeong-
dc.description.degreeMaster-
dc.citation.pages59-
dc.contributor.affiliation수의과대학 수의학과-
dc.date.awarded2013-02-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
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