Browse

Role of Vitamin D3 Up-regulated Protein 1(VDUP1) in Mouse Asthma Model

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
이보람
Advisor
김대용
Major
수의과대학 수의학과
Issue Date
2013-02
Publisher
서울대학교 대학원
Description
학위논문 (석사)-- 서울대학교 대학원 : 수의학과(수의병리학전공), 2013. 2. 김대용.
Abstract
Vitamin D3 upregulated protein 1 (VDUP1) is a multifunctional protein that is mainly distributed in immune organs and can exacerbate tumorigenesis and chronic inflammation, it may be related to an increase of immune-mediated materials such as proinflammatory cytokines, lymphokines, and transcription factors. In asthma, several cytokines have been reported to be involved in the inflammatory process. Here, we evaluated the effect of a lack of VDUP1 in ovalbumin (OVA)-induced airway inflammation in a mouse model of asthma. VDUP1 -/-(KO) and wild type (WT) mice were sensitized to OVA by intraperitoneal injection, and challenged with repeated exposure to OVA using an ultrasonic nebulizer. To investigate the difference between the groups, airway hyperresponsiveness (AHR), inflammatory cell number, and immunoglobulin E levels in bronchoalveolar lavage fluid (BALF), histopathological lesions (H&E stain), immunohistochemistry using inducible nitric oxide synthase (iNOS) antibody. And real-time polymerase chain reaction (PCR) using messenger RNA (mRNA) from lung tissue was analyzed. AHR, infiltration of leukocytes into the lung, total number of leukocytes recovered in BALF, and mucus secretion by goblet cells in bronchus were aggravated in KO/OVA mice compared with WT/OVA mice. The IgE level of BALF from KO/OVA treated mice was higher than that of WT/OVA mice. In lung sections, there was higher iNOS staining intensity in KO/OVA mice than WT/OVA mice. In addition, the mRNA of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-4, IL-1β, and iNOS increased in KO/OVA treated mice compared with WT/OVA mice. Depletion of VDUP1 aggravated the development of allergic airway inflammation and hyperresponsiveness possibly through various cytokines involved in VDUP1.
Language
English
URI
https://hdl.handle.net/10371/133693
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse